Effects of radiation on frequency of chromosomal aberrations and sister chromatid exchange in the benthic worm Neanthes arenaceodentata

Conference · Fri Apr 01 04:00:00 EST 1983

OSTI ID:6661008

Harrison, F L; Rice, Jr, D W; Moore, D H; Varela, M

Traditional bioassays are unsuitable for assessing sublethal effects of low levels of radioactivity because mortality and phenotypic responses are not anticipated. We compared the usefulness of chromosomal aberration (CA) and sister chromatid exchange (SCE) induction as measures of low-level radiation effects in a sediment-dwelling marine worm, Neanthes arenaceodentata. Newly hatched larvae were exposed to two radiation exposure regimes. Groups of 100 larvae were exposed to either x rays delivered at high dose rates (0.7 Gy min/sup -1/) or to /sup 60/Co gamma rays delivered at low dose rates (4.8 X 10/sup -5/ to 1.2 X 10/sup -1/ Gy h/sup -1/). After irradiation, the larvae were exposed to 3 X 10/sup -5/M bromodeoxyuridine (BrdUrd) for 28 h (x-ray-irradiated larvae) or for 54 h (/sup 60/Co-irradiated larvae). Slides of larval cells were prepared for observation of CAs and SCEs. Frequencies of CAs were determined in first division cells; frequencies of SCEs were determined in second division cells. Results from x-ray irradiation indicated that dose-related increases occur in chromosome and chromatid deletions, but an x-ray dose greater than or equal to 2 Gy was required to observe a significant increase. Worm larvae receiving /sup 60/Co irradiation showed elevated SCE frequencies; a significant increase in SCE frequency was observed at 0.6 Gy. 49 references, 2 figures.

• Research Organization: Lawrence Livermore National Lab., CA (USA); Environmental Protection Agency, Washington, DC (USA). Office of Radiation Programs
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6661008
• Report Number(s): UCRL-88645; CONF-830424-2; ON: DE84014520
• Country of Publication: United States
• Language: English

Related Subjects

560153* -- Radiation Effects on Animals-- Invertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANNELIDS
AQUATIC ORGANISMS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHROMOSOMAL ABERRATIONS
DATA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GAMMA RADIATION
INFORMATION
INVERTEBRATES
IONIZING RADIATIONS
IRRADIATION
LOW DOSE IRRADIATION
MUTATIONS
NUMERICAL DATA
RADIATION EFFECTS
RADIATIONS
SISTER CHROMATID EXCHANGES
X RADIATION