High-Resolution Field Asymmetric Waveform Ion Mobility Spectrometry Using New Planar Geometry Analyzers

Journal Article · Thu Jun 01 04:00:00 EDT 2006 · Analytical Chemistry, 78(11):3706-3714

DOI:https://doi.org/10.1021/ac052020v· OSTI ID:885457

Shvartsburg, Alexandre A; Li, Fumin; Tang, Keqi; Smith, Richard D

Field asymmetric waveform ion mobility spectrometry (FAIMS) has emerged as a powerful tool of broad utility for separation and characterization of gas-phase ions, especially in conjunction with mass spectrometry (MS). In FAIMS, ions are filtered by the dependence of mobility on electric field while carried by gas flow through the analytical gap between two electrodes of either planar (p-) or cylindrical (c-) geometry. Most FAIMS/MS systems employ c-FAIMS because of its ease of coupling to MS, yet the merits of two geometries have not been compared in detail. Here, a priori simulations have revealed that reducing the FAIMS curvature always improves resolution at equal sensitivity. In particular, the resolving power of p-FAIMS exceeds that of c-FAIMS, typically by a factor of 2 - 4 depending on the ion species and carrier gas. We have constructed a new planar FAIMS that incorporates a curtain plate interface for effective operation with an ESI ion source and is joined to MS using an ion funnel interface with a novel slit aperture. The resolution increases up to 4-fold over existing c-FAIMS, even though the analysis is ~3 times faster. This allows separation of species not feasible in previous FAIMS studies, e.g., protonated leucine and isoleucine or new bradykinin isomers. The improvement for protein conformers (of ubiquitin) is less significant, possibly because of multiple unresolved geometries.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 885457
• Report Number(s): PNNL-SA-47897; 400412000
• Journal Information: Analytical Chemistry, 78(11):3706-3714, Journal Name: Analytical Chemistry, 78(11):3706-3714 Journal Issue: 11 Vol. 78
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRADYKININ
ELECTRIC FIELDS
ELECTRODES
GAS FLOW
GEOMETRY
ION MOBILITY
ION SOURCES
ISOMERS
LEUCINE
MASS SPECTROSCOPY
PLATES
PROTEINS
RESOLUTION
SENSITIVITY
SPECTROSCOPY
WAVE FORMS