Method for loading lipsomes with ionizable phosphorylated hydrophobic compounds, pharmaceutical preparations and a method for administering the preparations

Mehlhorn, Rolf Joachim

Method for loading lipsomes with ionizable phosphorylated hydrophobic compounds, pharmaceutical preparations and a method for administering the preparations

Patent · Mon Oct 26 23:00:00 EST 1998

OSTI ID:879398

Mehlhorn, Rolf Joachim ^[1]

Richmond, CA

A method of entrapping ionizable compounds, preferably phosphorylated hydrophobic compounds, into liposomes having transmembrane gradients is disclosed. The procedures involve forming liposomes in an acidic medium or a basic medium, adding to the acidic medium a cationic compound or to the basic medium an anionic compound and then adding a base to the cationic-containing medium or an acid to the anionic-containing medium, thereby inducing the ionizable compound into the liposomes' internal aqueous phase. The compound-entrapped liposomes prepared in accordance with the disclosed methods may be used as pharmaceutical preparations. Methods of administering such pharmaceutical preparations are also disclosed.

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Research Organization:: Lawrence Berkeley National Laboratory

DOE Contract Number:: AC03-76SF00098

Assignee:: The Reagents of the University of California (Oakland, CA)

Patent Number(s):: US 5,827,532

Application Number:: 08/791557

OSTI ID:: 879398

Country of Publication:: United States

Language:: English

References (19)

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