Microfabricated sleeve devices for chemical reactions

Patent · Wed Jan 01 04:00:00 EST 2003

OSTI ID:875073

Northrup, M Allen ^[1]

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1. Berkeley, CA

A silicon-based sleeve type chemical reaction chamber that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The reaction chamber combines a critical ratio of silicon and non-silicon based materials to provide the thermal properties desired. For example, the chamber may combine a critical ratio of silicon and silicon nitride to the volume of material to be heated (e.g., a liquid) in order to provide uniform heating, yet low power requirements. The reaction chamber will also allow the introduction of a secondary tube (e.g., plastic) into the reaction sleeve that contains the reaction mixture thereby alleviating any potential materials incompatibility issues. The reaction chamber may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction. The reaction chamber may also be used in synthesis instruments, particularly those for DNA amplification and synthesis.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA
• DOE Contract Number: W-7405-ENG-48
• Assignee: The Regents of the University of California (Oakland, CA)
• Patent Number(s): US 6524532
• OSTI ID: 875073
• Country of Publication: United States
• Language: English

Related Subjects

/422/435/
alleviating
allow
amplification
andor
based
biochemical
bulk
bulk silicon
chain
chamber
chemical
chemical reaction
combine
combines
contains
convection
cooling
critical
devices
dna
doped
example
examples
heated
heaters
heating
incompatibility
inorganic
instruments
introduction
issues
ligase
liquid
material
materials
microfabricated
mixture
nitride
non-silicon
organic
pcr
plastic
polymerase
polysilicon
potential
power
processing
properties
provide
ratio
reaction
reaction chamber
reaction mixture
reactions
requirements
secondary
silicon
silicon nitride
silicon-based
sleeve
synthesis
synthetic
thermal
thermal-cycling-based
tube
type
uniform
utilized
volume