Use of continuous/contiguous stacking hybridization as a diagnostic tool

Patent · Mon Dec 31 23:00:00 EST 2001

OSTI ID:874683

Mirzabekov, Andrei Darievich ^[1]; Kirillov, Eugene Vladislavovich ^[1]; Parinov, Sergei Valeryevich ^[1]; Barski, Victor Evgenievich ^[1]; Dubiley, Svetlana Alekseevna ^[2]

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1. Moscow, RU
2. Kiev, RU

A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates. A method is also provided for determining the length of a repeat sequence in DNA or RNA, and also for determining the base sequence of unknown DNA or RNA.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL
• DOE Contract Number: W-31109-ENG-38
• Assignee: The University of Chicago (Chicago, IL)
• Patent Number(s): US 6440671
• OSTI ID: 874683
• Country of Publication: United States
• Language: English

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