Use of continuous/contiguous stacking hybridization as a diagnostic tool
- Moscow, RU
- Kiev, RU
A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates. A method is also provided for determining the length of a repeat sequence in DNA or RNA, and also for determining the base sequence of unknown DNA or RNA.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- DOE Contract Number:
- W-31109-ENG-38
- Assignee:
- The University of Chicago (Chicago, IL)
- Patent Number(s):
- US 6440671
- OSTI ID:
- 874683
- Country of Publication:
- United States
- Language:
- English
A method for DNA sequencing by hybridization with oligonucleotide matrix
|
journal | January 1991 |
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Use of continuous/contiguous stacking hybridization as a diagnostic tool
Use of continuous/contiguous stacking hybridization as a diagnostic tool
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stacking
hybridization
diagnostic
tool
method
detecting
disease-associated
alleles
patient
genetic
material
provided
oligonucleotide
molecules
synthesized
compliment
base
sequences
disease
associated
immobilized
predetermined
position
substrate
contacted
form
duplexes
extend
length
tagged
incorporate
universal
bases
mixture
guanine
cytosine
thymine
adenine
complementary
nucleotide
strands
fluorochrome
radiates
light
wavelength
treated
washed
patterns
radiating
compared
diseases
prepared
identical
substrates
determining
repeat
sequence
dna
rna
base sequence
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