Use of continuous/contiguous stacking hybridization as a diagnostic tool

Patent · Fri Jan 01 00:00:00 EST 1999

OSTI ID:872311

Mirzabekov, Andrei Darievich ^[1]; Yershov, Gennadiy Moseyevich ^[2]; Kirillov, Eugene Vladislavovich ^[1]; Parinov, Sergei Valeryevich ^[1]; Barski, Victor Evgenievich ^[1]; Lysov, Yuri Petrovich ^[1]

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1. Moscow, RU
2. Zelenograd, RU

A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States)
• DOE Contract Number: W-31109-ENG-38
• Assignee: University of Chicago (Chicago, IL)
• Patent Number(s): US 5908745
• OSTI ID: 872311
• Country of Publication: United States
• Language: English

Related Subjects

continuous
contiguous
stacking
hybridization
diagnostic
tool
method
detecting
disease-associated
alleles
patient
genetic
material
provided
whereby
oligonucleotide
molecules
synthesized
compliment
base
sequences
disease
associated
immobilized
predetermined
position
substrate
contacted
form
duplexes
extend
length
tagged
incorporate
universal
bases
mixture
guanine
cytosine
thymine
adenine
complementary
nucleotide
strands
fluorochrome
radiates
light
wavelength
treated
washed
patterns
radiating
therefrom
compared
various
diseases
prepared
identical
substrates
various diseases
predetermined position
light pattern
base sequences
predetermined wavelength
genetic material
provided whereby
oligonucleotide molecules
base sequence
light patterns
complementary nucleotide
patient genetic
nucleotide strand
nucleotide strands
compliment base
diagnostic tool
treated substrate
stacking hybridization
associated alleles
detecting disease-associated
disease-associated alleles
contiguous stacking
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