Micromechanical antibody sensor

Title: Micromechanical antibody sensor

Full Record
Other Related Research

Abstract

A sensor apparatus is provided using a microcantilevered spring element having a coating of a detector molecule such as an antibody or antigen. A sample containing a target molecule or substrate is provided to the coating. The spring element bends in response to the stress induced by the binding which occurs between the detector and target molecules. Deflections of the cantilever are detected by a variety of detection techniques. The microcantilever may be approximately 1 to 200 .mu.m long, approximately 1 to 50 .mu.m wide, and approximately 0.3 to 3.0 .mu.m thick. A sensitivity for detection of deflections is in the range of 0.01 nanometers.

Inventors:

Thundat, Thomas G ^[1]; Jacobson, K Bruce ^[2]; Doktycz, Mitchel J ^[1]; Kennel, Stephen J ^[2]; Warmack, Robert J ^[1]

Knoxville, TN
Oak Ridge, TN

Publication Date:: 2001-01-01

Research Org.:: LOCKHEED MARTIN ENERGY RES COR

OSTI Identifier:: 873996

Patent Number(s):: US 6289717

Assignee:: U. T. Battelle, LLC (Oak Ridge, TN)

DOE Contract Number:: AC05-96OR22464

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: micromechanical; antibody; sensor; apparatus; provided; microcantilevered; spring; element; coating; detector; molecule; antigen; sample; containing; target; substrate; bends; response; stress; induced; binding; occurs; molecules; deflections; cantilever; detected; variety; detection; techniques; microcantilever; approximately; 200; 50; wide; thick; sensitivity; range; 01; nanometers; detection techniques; spring element; sample containing; sensor apparatus; stress induced; microcantilevered spring; target molecule; /73/422/436/

Citation Formats


                    Thundat, Thomas G, Jacobson, K Bruce, Doktycz, Mitchel J, Kennel, Stephen J, and Warmack, Robert J. Micromechanical antibody sensor.  United States: N. p., 2001. 
        Web.

Copy to clipboard


                    Thundat, Thomas G, Jacobson, K Bruce, Doktycz, Mitchel J, Kennel, Stephen J, & Warmack, Robert J. Micromechanical antibody sensor.  United States.

Copy to clipboard


                    Thundat, Thomas G, Jacobson, K Bruce, Doktycz, Mitchel J, Kennel, Stephen J, and Warmack, Robert J. Mon .  
        "Micromechanical antibody sensor".  United States.  https://www.osti.gov/servlets/purl/873996.

Copy to clipboard


                    
@article{osti_873996,

  title        = {Micromechanical antibody sensor},

  author       = {Thundat, Thomas G and Jacobson, K Bruce and Doktycz, Mitchel J and Kennel, Stephen J and Warmack, Robert J},

  abstractNote = {A sensor apparatus is provided using a microcantilevered spring element having a coating of a detector molecule such as an antibody or antigen. A sample containing a target molecule or substrate is provided to the coating. The spring element bends in response to the stress induced by the binding which occurs between the detector and target molecules. Deflections of the cantilever are detected by a variety of detection techniques. The microcantilever may be approximately 1 to 200 .mu.m long, approximately 1 to 50 .mu.m wide, and approximately 0.3 to 3.0 .mu.m thick. A sensitivity for detection of deflections is in the range of 0.01 nanometers.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/873996},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2001},

  month        = {1}

}

Copy to clipboard

Patent:

View Patent

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Micromechanical calorimetric sensor

Patent Thundat, Thomas G ; Doktycz, Mitchel J

A calorimeter sensor apparatus is developed utilizing microcantilevered spring elements for detecting thermal changes within a sample containing biomolecules which undergo chemical and biochemical reactions. The spring element includes a bimaterial layer of chemicals on a coated region on at least one surface of the microcantilever. The chemicals generate a differential thermal stress across the surface upon reaction of the chemicals with an analyte or biomolecules within the sample due to the heat of chemical reactions in the sample placed on the coated region. The thermal stress across the spring element surface creates mechanical bending of the microcantilever. The springmore »« less
Full Text Available
Micromechanical potentiometric sensors

Patent Thundat, Thomas G

A microcantilever potentiometric sensor utilized for detecting and measuring physical and chemical parameters in a sample of media is described. The microcantilevered spring element includes at least one chemical coating on a coated region, that accumulates a surface charge in response to hydrogen ions, redox potential, or ion concentrations in a sample of the media being monitored. The accumulation of surface charge on one surface of the microcantilever, with a differing surface charge on an opposing surface, creates a mechanical stress and a deflection of the spring element. One of a multitude of deflection detection methods may include the usemore »« less
Full Text Available
Micromechanical potentiometric sensors

Patent Thundat, T G

A microcantilever potentiometric sensor utilized for detecting and measuring physical and chemical parameters in a sample of media is described. The microcantilevered spring element includes at least one chemical coating on a coated region, that accumulates a surface charge in response to hydrogen ions, redox potential, or ion concentrations in a sample of the media being monitored. The accumulation of surface charge on one surface of the microcantilever, with a differing surface charge on an opposing surface, creates a mechanical stress and a deflection of the spring element. One of a multitude of deflection detection methods may include the usemore »« less
Bio-Chemo-Opto-Mechanical (BioCOM) Sensors for Real-Time Characterization for D&D Applications

Technical Report Thundat, Thomas

Basic and applied research will be conducted to develop simple to use chemical and biological sensor chips utilizing bio-chemo-mechanics for real-time, in-situ, detection of technetium, mercury, uranium, and beryllium for deactivation and decommissioning applications. The bio-chemo-opto-mechanical (Bio-COM) chip involves properly fashioned arrays of micromachined silicon cantilevers containing embedded deformable diffraction gratings functionalized with chemically selective coatings. Adsorption of specific molecules on the cantilever array leads to bending, which changes the diffraction of light from the array. The bio-chemo-opto-mechanical (BioCOM) chips will be designed to contain an array of pixels, with each pixel containing an array of microcantilever springs in whichmore »« less
https://doi.org/10.2172/835394

Full Text Available
Bio-Chemo-Opto-Mechanical (BioCOM) Sensors for Real-Time Characterization for D&D Applications (82799)

Technical Report Thundat, Thomas

Basic and applied research is being conducted to develop simple to use chemical and biological sensor chips utilizing bio-chemo-mechanics for real-time, in-situ, detection of technetium, mercury, uranium, copper, and lead for deactivation and decommissioning applications. The bio-chemo-opto-mechanical (Bio-COM) chip involves properly fashioned arrays of micromachined silicon cantilevers containing embedded deformable diffraction gratings functionalized with chemically selective coatings. Adsorption of specific molecules on the cantilever array leads to bending, which changes the diffraction of light from the array. The biochemo- opto-mechanical (BioCOM) chips will be designed to contain an array of pixels, with each pixel containing an array of microcantilever springsmore »« less
https://doi.org/10.2172/835396

Full Text Available

Similar Records