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Title: Advanced surface-enhanced Raman gene probe systems and methods thereof

Abstract

The subject invention is a series of methods and systems for using the Surface-Enhanced Raman (SER)-labeled Gene Probe for hybridization, detection and identification of SER-labeled hybridized target oligonucleotide material comprising the steps of immobilizing SER-labeled hybridized target oligonucleotide material on a support means, wherein the SER-labeled hybridized target oligonucleotide material comprise a SER label attached either to a target oligonucleotide of unknown sequence or to a gene probe of known sequence complementary to the target oligonucleotide sequence, the SER label is unique for the target oligonucleotide strands of a particular sequence wherein the SER-labeled oligonucleotide is hybridized to its complementary oligonucleotide strand, then the support means having the SER-labeled hybridized target oligonucleotide material adsorbed thereon is SERS activated with a SERS activating means, then the support means is analyzed.

Inventors:
 [1]
  1. Knoxville, TN
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
OSTI Identifier:
873496
Patent Number(s):
US 6174677
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
advanced; surface-enhanced; raman; probe; systems; methods; subject; series; ser; -labeled; hybridization; detection; identification; ser-labeled; hybridized; target; oligonucleotide; material; comprising; steps; immobilizing; support; means; comprise; label; attached; sequence; complementary; unique; strands; particular; strand; adsorbed; thereon; sers; activated; activating; analyzed; oligonucleotide strand; hybridized target; surface-enhanced raman; nucleotide sequence; material comprising; support means; enhanced raman; oligonucleotide strands; adsorbed thereon; nucleotide strand; nucleotide strands; /435/356/

Citation Formats

Vo-Dinh, Tuan. Advanced surface-enhanced Raman gene probe systems and methods thereof. United States: N. p., 2001. Web.
Vo-Dinh, Tuan. Advanced surface-enhanced Raman gene probe systems and methods thereof. United States.
Vo-Dinh, Tuan. 2001. "Advanced surface-enhanced Raman gene probe systems and methods thereof". United States. https://www.osti.gov/servlets/purl/873496.
@article{osti_873496,
title = {Advanced surface-enhanced Raman gene probe systems and methods thereof},
author = {Vo-Dinh, Tuan},
abstractNote = {The subject invention is a series of methods and systems for using the Surface-Enhanced Raman (SER)-labeled Gene Probe for hybridization, detection and identification of SER-labeled hybridized target oligonucleotide material comprising the steps of immobilizing SER-labeled hybridized target oligonucleotide material on a support means, wherein the SER-labeled hybridized target oligonucleotide material comprise a SER label attached either to a target oligonucleotide of unknown sequence or to a gene probe of known sequence complementary to the target oligonucleotide sequence, the SER label is unique for the target oligonucleotide strands of a particular sequence wherein the SER-labeled oligonucleotide is hybridized to its complementary oligonucleotide strand, then the support means having the SER-labeled hybridized target oligonucleotide material adsorbed thereon is SERS activated with a SERS activating means, then the support means is analyzed.},
doi = {},
url = {https://www.osti.gov/biblio/873496}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}

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