Advanced surface-enhanced Raman gene probe systems and methods thereof

Vo-Dinh, Tuan

Advanced surface-enhanced Raman gene probe systems and methods thereof

Patent · Mon Jan 01 04:00:00 EST 2001

OSTI ID:873496

Vo-Dinh, Tuan ^[1]

Knoxville, TN

The subject invention is a series of methods and systems for using the Surface-Enhanced Raman (SER)-labeled Gene Probe for hybridization, detection and identification of SER-labeled hybridized target oligonucleotide material comprising the steps of immobilizing SER-labeled hybridized target oligonucleotide material on a support means, wherein the SER-labeled hybridized target oligonucleotide material comprise a SER label attached either to a target oligonucleotide of unknown sequence or to a gene probe of known sequence complementary to the target oligonucleotide sequence, the SER label is unique for the target oligonucleotide strands of a particular sequence wherein the SER-labeled oligonucleotide is hybridized to its complementary oligonucleotide strand, then the support means having the SER-labeled hybridized target oligonucleotide material adsorbed thereon is SERS activated with a SERS activating means, then the support means is analyzed.

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Research Organization:: LOCKHEED MARTIN ENRGY SYST INC

DOE Contract Number:: AC05-84OR21400

Assignee:: UT-Battelle, LLC (Oak Ridge, TN)

Patent Number(s):: US 6174677

OSTI ID:: 873496

Country of Publication:: United States

Language:: English

References (16)

Surface-enhanced Raman spectroscopy using metallic nanostructures1The submitted manuscript has been authored by a contractor of the U.S Government under contract No. DE-AC05-96OR22464. Accordingly, the U.S. Government retains a nonexclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others to do so, for U.S. Government purposes.1 Vo-Dinh, Tuan TrAC Trends in Analytical Chemistry, Vol. 17, Issue 8-9 https://doi.org/10.1016/S0165-9936(98)00069-7	journal	August 1998
Surface raman spectroelectrochemistry: Part I. Heterocyclic, aromatic, and aliphatic amines adsorbed on the anodized silver electrode Jeanmaire, David L.; Van Duyne, Richard P. Journal of Electroanalytical Chemistry and Interfacial Electrochemistry, Vol. 84, Issue 1, p. 1-20 https://doi.org/10.1016/S0022-0728(77)80224-6	journal	November 1977
Structure of Pna-Nucleic Acid Complexes Eriksson, Magdalena Nucleosides and Nucleotides, Vol. 16, Issue 5-6 https://doi.org/10.1080/07328319708002925	journal	May 1997
Surface-Enhanced Raman Analysis of p -Nitroaniline on Vacuum Evaporation and Chemically Deposited Silver-Coated Alumina Substrates Li, Ying-Sing; Vo-Dinh, Tuan; Stokes, David L. Applied Spectroscopy, Vol. 46, Issue 9 https://doi.org/10.1366/0003702924123674	journal	September 1992
Derivatization of unprotected polynucleotides Chu, Barbara C. F.; Wahl, Geoffrey M.; Orgel, Leslie E. Nucleic Acids Research, Vol. 11, Issue 18 https://doi.org/10.1093/nar/11.18.6513	journal	January 1983
Analytical strategies for the use of DNA probes Matthews, Jayne A.; Kricka, Larry J. Analytical Biochemistry, Vol. 169, Issue 1, p. 1-25 https://doi.org/10.1016/0003-2697(88)90251-5	journal	February 1988
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Human glucokinase gene: isolation, characterization, and identification of two missense mutations linked to early-onset non-insulin-dependent (type 2) diabetes mellitus. Stoffel, M.; Froguel, P.; Takeda, J. Proceedings of the National Academy of Sciences, Vol. 89, Issue 16 https://doi.org/10.1073/pnas.89.16.7698	journal	August 1992
Charge-Coupled Device Fluorescence Detection for Capillary-Zone Electrophoresis (CCD-CZE) Cheng, Yung-Fong; Piccard, Richard D.; Vo-Dinh, Tuan Applied Spectroscopy, Vol. 44, Issue 5 https://doi.org/10.1366/0003702904087082	journal	June 1990
A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1 Miki, Y.; Swensen, J.; Shattuck-Eidens, D. Science, Vol. 266, Issue 5182 https://doi.org/10.1126/science.7545954	journal	October 1994
Surface-Enhanced Raman Scattering Vo-Dinh, Tuan Photonic Probes of Surfaces, p. 65-95 https://doi.org/10.1016/B978-0-444-82198-0.50009-6	book	January 1995
Selective surface-enhanced Raman spectroscopy using a polymer-coated substrate Pal, Anjali.; Stokes, David L.; Alarie, Jean Pierre. Analytical Chemistry, Vol. 67, Issue 18 https://doi.org/10.1021/ac00114a009	journal	September 1995
Surface-Enhanced Raman Gene Probes Vo-Dinh, T.; Houck, K.; Stokes, D. L. Analytical Chemistry, Vol. 66, Issue 20 https://doi.org/10.1021/ac00092a014	journal	October 1994
Early-onset Alzheimer's disease caused by mutations at codon 717 of the β-amyloid precursor protein gene Chartier-Harlin, Marie-Christine; Crawford, Fiona; Houlden, Henry Nature, Vol. 353, Issue 6347 https://doi.org/10.1038/353844a0	journal	October 1991
A multi‐optical‐fiber array with charge‐coupled device image detection for parallel processing of light signals and spectra Piccard, Richard D.; Vo‐Dinh, Tuan Review of Scientific Instruments, Vol. 62, Issue 3 https://doi.org/10.1063/1.1142110	journal	March 1991
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Advanced surface-enhanced Raman gene probe systems and methods thereof

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