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Surface enhanced Raman gene probe and methods thereof

Patent ·
OSTI ID:871728
 [1]
  1. Knoxville, TN
+ Show Author Affiliations

The subject invention disclosed herein is a new gene probe biosensor and methods thereof based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays.

Research Organization:
LOCKHEED MARTIN ENRGY SYST INC
DOE Contract Number:
AC05-84OR21400
Assignee:
Lockheed Martin Energy Research Corporation (Oak Ridge, TN)
Patent Number(s):
US 5783389
OSTI ID:
871728
Country of Publication:
United States
Language:
English

References (6)

Surface raman spectroelectrochemistry: Part I. Heterocyclic, aromatic, and aliphatic amines adsorbed on the anodized silver electrode journal November 1977
A rapid semi-automated microtiter plate method for analysis and sequencing by PCR from bacterial stocks journal January 1989
Charge-Coupled Device Fluorescence Detection for Capillary-Zone Electrophoresis (CCD-CZE) journal June 1990
Selective surface-enhanced Raman spectroscopy using a polymer-coated substrate journal September 1995
Surface-Enhanced Raman Gene Probes journal October 1994
Wet chemical approaches to the characterization of organic surfaces: self-assembled monolayers, wetting, and the physical-organic chemistry of the solid-liquid interface journal January 1990

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