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Title: Surface enhanced Raman gene probe and methods thereof

Patent ·
OSTI ID:871869
 [1]
  1. Knoxville, TN
+ Show Author Affiliations

The subject invention disclosed herein is a new gene probe biosensor and methods thereof based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
DOE Contract Number:
AC05-84OR21400
Assignee:
Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN)
Patent Number(s):
US 5814516
OSTI ID:
871869
Country of Publication:
United States
Language:
English

References (5)

Surface raman spectroelectrochemistry: Part I. Heterocyclic, aromatic, and aliphatic amines adsorbed on the anodized silver electrode journal November 1977
Wet chemical approaches to the characterization of organic surfaces: self-assembled monolayers, wetting, and the physical-organic chemistry of the solid-liquid interface journal January 1990
Surface-Enhanced Raman Gene Probes journal October 1994
Charge-Coupled Device Fluorescence Detection for Capillary-Zone Electrophoresis (CCD-CZE) journal June 1990
Selective surface-enhanced Raman spectroscopy using a polymer-coated substrate journal September 1995

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Related Subjects

surface
enhanced
raman
probe
methods
subject
disclosed
biosensor
based
scattering
sers
label
detection
ser
comprises
support
means
oligonucleotide
strand
labeled
active
substrate
disposed
probes
adsorbed
thereon
biotargets
bacterial
viral
dna
rna
pna
detected
via
hybridization
strands
complementary
supporting
fiberoptic
array
performance
multiple
assays
waveguide
microsensor
charge-coupled
devices
photodiode
arrays
sers active
probe biosensor
oligonucleotide strand
sers label
active substrate
diode arrays
photodiode array
charge-coupled device
means supporting
photodiode arrays
substrate disposed
sensor array
coupled device
raman scattering
support means
diode array
sensor comprises
enhanced raman
optic probe
surface enhanced
fiberoptic probe
waveguide microsensor
strand labeled
strands complementary
viral dna
via hybridization
probes adsorbed
microsensor array
multiple assays
oligonucleotide strands
biosensor comprises
charge-coupled devices
adsorbed thereon
probe via
nucleotide strand
nucleotide strands
fiberoptic probes
label detection
optic probes
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