Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase

Srienc, Friedrich; Jackson, John K; Somers, David A

Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase

Patent · Sat Jan 01 04:00:00 EST 2000

OSTI ID:873364

Srienc, Friedrich ^[1]; Jackson, John K ^[2]; Somers, David A ^[3]

Lake Elmo, MN
Plymouth, MN
Roseville, MN

A genetically engineered Pseudomonas oleovorans phaC1 polyhydroxyalkanoate (PHA) polymerase having tailored substrate specificity is provided. The modified PHA polymerase is preferably a "bispecific" PHA polymerase capable of copolymerizing a short chain length monomer and a medium chain length monomer is provided. Methods for making the modified PHA polymerase and for making nucleic acids encoding the modified PHA polymerase are also disclosed, as are methods of producing PHA using the modified PHA polymerase. The invention further includes methods to assay for altered substrate specificity.

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Research Organization:: The Consortium for Plant Biotechnology Research, Inc., West Lafayette, IN (US)

DOE Contract Number:: FC05-92OR22072

Assignee:: Regents of University of Minnesota (Minneapolis, MN)

Patent Number(s):: US 6143952

OSTI ID:: 873364

Country of Publication:: United States

Language:: English

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/800/435/999/
acids
acids encoding
altered
assay
bispecific
capable
chain
chain length
copolymerizing
disclosed
encoding
engineered
genetically
genetically engineered
length
medium
methods
modified
monomer
nucleic
nucleic acid
nucleic acids
oleovorans
oleovorans phac1
pha
pha polymerase
phac1
polyhydroxyalkanoate
polymerase
preferably
producing
provided
pseudomonas
pseudomonas oleovorans
specificity
substrate
tailored

Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase

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