Polyhydroxyalkanoate synthesis in plants

Srienc, Friedrich; Somers, David A; Hahn, J J; Eschenlauer, Arthur C

Polyhydroxyalkanoate synthesis in plants

Patent · Sat Jan 01 04:00:00 EST 2000

OSTI ID:873164

Srienc, Friedrich ^[1]; Somers, David A ^[2]; Hahn, J J ^[3]; Eschenlauer, Arthur C ^[4]

Lake Elmo, MN
Roseville, MN
New Brighton, MN
Circle Pines, MN

Novel transgenic plants and plant cells are capable of biosynthesis of polyhydroxyalkanoate (PHA). Heterologous enzymes involved in PHA biosynthesis, particularly PHA polymerase, are targeted to the peroxisome of a transgenic plant. Transgenic plant materials that biosynthesize short chain length monomer PHAs in the absence of heterologous .beta.-ketothiolase and acetoacetyl-CoA reductase are also disclosed.

View Patent

Research Organization:: The Consortium for Plant Biotechnology Research, Inc., West Lafayette, IN (US)

DOE Contract Number:: FC05-92OR22072

Assignee:: Regents of University of Minnesota (Minneapolis, MN)

Patent Number(s):: US 6103956

OSTI ID:: 873164

Country of Publication:: United States

Language:: English

References (42)

Gas chromatographic determination of poly-β-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis Riis, V.; Mai, W. Journal of Chromatography A, Vol. 445 https://doi.org/10.1016/S0021-9673(01)84535-0	journal	January 1988
Genetically transformed maize plants from protoplasts Rhodes, C.; Pierce, D.; Mettler, I. Science, Vol. 240, Issue 4849 https://doi.org/10.1126/science.2832947	journal	April 1988
Characterization of two 3-ketothiolases possessing differing substrate specificities in the polyhydroxyalkanoate synthesizing organism Alcaligenes eutrophus Haywood, G. W.; Anderson, A. J.; Chu, L. FEMS Microbiology Letters, Vol. 52, Issue 1-2 https://doi.org/10.1111/j.1574-6968.1988.tb02577.x	journal	July 1988
Polyhydroxybutyrate, a Biodegradable Thermoplastic, Produced in Transgenic Plants Poirier, Y.; Dennis, D. E.; Klomparens, K. Science, Vol. 256, Issue 5056 https://doi.org/10.1126/science.256.5056.520	journal	April 1992
Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA Huisman, G. W.; Wonink, E.; Meima, R. Journal of Biological Chemistry, Vol. 266, Issue 4 https://doi.org/10.1016/S0021-9258(18)52227-4	journal	February 1991
Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Ostle, A. G.; Holt, J. G. Applied and Environmental Microbiology, Vol. 44, Issue 1 https://doi.org/10.1128/AEM.44.1.238-241.1982	journal	January 1982
The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector Koncz, Csaba; Schell, Jeff MGG Molecular & General Genetics, Vol. 204, Issue 3 https://doi.org/10.1007/BF00331014	journal	September 1986
Expression of genes transferred into monocot and dicot plant cells by electroporation. Fromm, M.; Taylor, L. P.; Walbot, V. Proceedings of the National Academy of Sciences, Vol. 82, Issue 17, p. 5824-5828 https://doi.org/10.1073/pnas.82.17.5824	journal	September 1985
Purification and properties of ?-ketothiolase from Zoogloea ramigera Nishimura, Takeko; Saito, Terumi; Tomita, Kenkichi Archives of Microbiology, Vol. 116, Issue 1 https://doi.org/10.1007/BF00408729	journal	January 1978
Microbodies—Peroxisomes and Glyoxysomes Tolbert, N. E. The Plant Cell https://doi.org/10.1016/B978-0-12-675401-8.50015-4	book	January 1980
Production of Polyhydroxyalkanoates, a Family of Biodegradable Plastics and Elastomers, in Bacteria and Plants Poirier, Yves; Nawrath, Christianae; Somerville, Chris Nature Biotechnology, Vol. 13, Issue 2, p. 142-150 https://doi.org/10.1038/nbt0295-142	journal	February 1995
Plant Organellar Targeting Sequences Wallace, T. P.; Howe, C. J. Plant Molecular Biology Labfax https://doi.org/10.1016/B978-0-08-091753-5.50014-7	book	January 1993
Biogenesis of Peroxisomes Lazarow, P. B.; Fujiki, Y. Annual Review of Cell Biology, Vol. 1, Issue 1 https://doi.org/10.1146/annurev.cb.01.110185.002421	journal	November 1985
A Pseudomonas strain accumulating polyesters of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids Steinb�chel, Alexander; Wiese, Stefan Applied Microbiology and Biotechnology, Vol. 37, Issue 6 https://doi.org/10.1007/BF00174829	journal	September 1992
Production of heteropolymeric polyhydroxyalkanoate in Eschericha coli from a single carbon source Eschenlauer, Arthur C.; Stoup, Sandra K.; Srienc, Friedrich International Journal of Biological Macromolecules, Vol. 19, Issue 2 https://doi.org/10.1016/0141-8130(96)01114-2	journal	August 1996
An NADP-linked acetoacetyl CoA reductase from Zoogloea ramigera Saito, Terumi; Fukui, Tetsuya; Ikeda, Fumiaki Archives of Microbiology, Vol. 114, Issue 3 https://doi.org/10.1007/BF00446864	journal	January 1977
Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16 Peoples, O. P.; Sinskey, A. J. Journal of Biological Chemistry, Vol. 264, Issue 26 https://doi.org/10.1016/S0021-9258(19)84825-1	journal	September 1989
Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation. Nawrath, C.; Poirier, Y.; Somerville, C. Proceedings of the National Academy of Sciences, Vol. 91, Issue 26 https://doi.org/10.1073/pnas.91.26.12760	journal	December 1994
Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants Fromm, Michael E.; Morrish, Fionnuala; Armstrong, Charles Nature Biotechnology, Vol. 8, Issue 9 https://doi.org/10.1038/nbt0990-833	journal	September 1990
Catalase Aebi, Hugo Methods of Enzymatic Analysis https://doi.org/10.1016/B978-0-12-091302-2.50032-3	book	January 1974
Production of a novel copolyester of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids by Pseudomonas sp. 61-3 from sugars Kato, M.; Bao, H. J.; Kang, C. -K. Applied Microbiology and Biotechnology, Vol. 45, Issue 3 https://doi.org/10.1007/s002530050697	journal	April 1996
One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Chung, C. T.; Niemela, S. L.; Miller, R. H. Proceedings of the National Academy of Sciences, Vol. 86, Issue 7 https://doi.org/10.1073/pnas.86.7.2172	journal	April 1989
Saccharomyces cerevisiae expressing bacterial polyhydroxybutyrate synthase produces poly-3-hydroxybutyrate Leaf, T. A.; Peterson, M. S.; Stoup, S. K. Microbiology, Vol. 142, Issue 5 https://doi.org/10.1099/13500872-142-5-1169	journal	May 1996
Microbodies in Higher Plants Beevers, H. Annual Review of Plant Physiology, Vol. 30, Issue 1 https://doi.org/10.1146/annurev.pp.30.060179.001111	journal	June 1979
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Murashige, Toshio; Skoog, Folke Physiologia Plantarum, Vol. 15, Issue 3, p. 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x	journal	July 1962
Fatty acid degradation in plant peroxisomes: Function and biosynthesis of the enzymes involved Kindl, H. Biochimie, Vol. 75, Issue 3-4 https://doi.org/10.1016/0300-9084(93)90080-C	journal	January 1993
Metabolism in Plant Peroxisomes Huang, Anthony H. C. Cellular and Subcellular Localization in Plant Metabolism https://doi.org/10.1007/978-1-4757-4727-0_3	book	January 1982
Use of paromomycin as a selective agent for oat transformation Torbert, Kimberly A.; Rines, Howard W.; Somers, David A. Plant Cell Reports, Vol. 14, Issue 10 https://doi.org/10.1007/BF00232728	journal	July 1995
Stable transformation of maize after gene transfer by electroporation Fromm, Michael E.; Taylor, Loverine P.; Walbot, Virginia Nature, Vol. 319, Issue 6056 https://doi.org/10.1038/319791a0	journal	February 1986
Bacterial polyhydroxyalkanoates Lee, Sang Yup Biotechnology and Bioengineering, Vol. 49, Issue 1 https://doi.org/10.1002/(SICI)1097-0290(19960105)49:1<1::AID-BIT1>3.0.CO;2-P	journal	March 2000
Poly(β-hydroxyalkanoate) copolymers containing brominated repeating units produced by Pseudomonas oleovorans Kim, Young B.; Lenz, R. W.; Fuller, R. Clinton Macromolecules, Vol. 25, Issue 7 https://doi.org/10.1021/ma00033a002	journal	March 1992
Intracellular pH in singleSaccharomyces cerevisiae cells Hernlem, B. J.; Srienc, F. Biotechnology Techniques, Vol. 3, Issue 2 https://doi.org/10.1007/BF01875557	journal	March 1989
Bacteriophage lambda cloning system for the construction of directional cDNA libraries. Meissner, P. S.; Sisk, W. P.; Berman, M. L. Proceedings of the National Academy of Sciences, Vol. 84, Issue 12 https://doi.org/10.1073/pnas.84.12.4171	journal	June 1987
Growth Kinetics, Nutrient Uptake, and Expression of the Alcaligenes eutrophus Poly(β-hydroxybutyrate) Synthesis Pathway in Transgenic Maize Cell Suspension Cultures Hahn, J. J.; Eschenlauer, A. C.; Narrol, M. H. Biotechnology Progress, Vol. 13, Issue 4 https://doi.org/10.1021/bp970033r	journal	August 1997
Manipulation of the genes for poly-?-hydroxybutyric acid synthesis in Alcaligenes eutrophus Park, Hae-Chul; Park, Jin-Seo; Lee, Yong-Hyun Biotechnology Letters, Vol. 17, Issue 7 https://doi.org/10.1007/BF00130359	journal	July 1995
Plastics from bacteria and for bacteria: Poly(β-hydroxyalkanoates) as natural, biocompatible, and biodegradable polyesters Brandl, Helmut; Gross, Richard A.; Lenz, Robert W. Microbial Bioproducts https://doi.org/10.1007/BFb0010232	book
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Laemmli, U. K. Nature, Vol. 227, Issue 5259, p. 680-685 https://doi.org/10.1038/227680a0	journal	August 1970
Modification of Plant Lipid Synthesis Topfer, R.; Martini, N.; Schell, J. Science, Vol. 268, Issue 5211 https://doi.org/10.1126/science.268.5211.681	journal	May 1995
Overexpression and Purification of the Soluble Polyhydroxyalkanoate Synthase from Alcaligenes eutrophus: Evidence for a Required Posttranslational Modification for Catalytic Activity Gerngross, T. U.; Snell, K. D.; Peoples, O. P. Biochemistry, Vol. 33, Issue 31 https://doi.org/10.1021/bi00197a035	journal	August 1994
Agrobacterium-Mediated Plant Transformation and its Further Applications to Plant Biology Klee, H.; Horsch, R.; Rogers, S. Annual Review of Plant Physiology, Vol. 38, Issue 1 https://doi.org/10.1146/annurev.pp.38.060187.002343	journal	June 1987
The carboxy-terminal end of glycolate oxidase directs a foreign protein into tobacco leaf peroxisomes. Volokita, M. The Plant Journal, Vol. 1, Issue 3 https://doi.org/10.1046/j.1365-313X.1991.t01-4-00999.x	journal	November 1991
Polyhydroxyalkanoic acids Steinbüchel, Alexander Biomaterials https://doi.org/10.1007/978-1-349-11167-1_3	book	January 1991

Similar Records

Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase

Patent · Fri Dec 31 23:00:00 EST 1999 · OSTI ID:873364

Peroxisomal. beta. -oxidation enzyme proteins in adrenoleukodystrophy: distinction between x-linked adrenoleukodystrophy and neonatal adrenoleukodystrophy

Journal Article · Sat Feb 28 23:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:6125742

Formation of D-3-hydroxybutyryl-coenzyme A by an acetoacetyl-coenzyme A reductase in Syntrophomonas wolfei subsp. wolfei

Journal Article · Thu Dec 30 23:00:00 EST 1993 · Archives of Microbiology · OSTI ID:514560

Related Subjects

-ketothiolase
/800/435/
absence
acetoacetyl-coa
beta
biosynthesis
biosynthesize
capable
cells
chain
chain length
disclosed
enzymes
heterologous
involved
length
materials
monomer
novel
particularly
peroxisome
pha
pha polymerase
phas
plant
plant cell
plant cells
plants
polyhydroxyalkanoate
polymerase
reductase
synthesis
targeted
transgenic
transgenic plant
transgenic plants

Polyhydroxyalkanoate synthesis in plants

Citation Formats

References (42)

Similar Records

Related Subjects