Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer

Kwok, Pui-Yan; Chen, Xiangning

Title: Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer

Patent · Fri Jan 01 00:00:00 EST 1999

OSTI ID:872487

Kwok, Pui-Yan ^[1]; Chen, Xiangning ^[2]

Clayton, MO
St. Louis, MO

A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.

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Research Organization:: Univ. of Washington, Seattle, WA (United States)

DOE Contract Number:: FG06-94ER61909

Assignee:: Washington University (St. Louis, MO)

Patent Number(s):: US 5945283

OSTI ID:: 872487

Country of Publication:: United States

Language:: English

References (11)

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Related Subjects

methods
kits
nucleic
acid
analysis
fluorescence
resonance
energy
transfer
method
detecting
presence
target
nucleotide
sequence
nucleotides
disclosed
comprised
forming
oligonucleotide
labeled
fluorophores
site
doubly
formed
addition
singly
dideoxynucleoside
triphosphate
polynucleotide
ligation
polynucleotides
detection
denaturation
indicates
provided
particularly
applicable
genotyping
resonance energy
energy transfer
particularly applicable
nucleic acid
target nucleotide
target site
acid target
nucleoside triphosphate
/435/436/

Title: Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer

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References (11)

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