Method for nucleic acid isolation using supercritical fluids

Nivens, David E; Applegate, Bruce M

Method for nucleic acid isolation using supercritical fluids

Patent · 01 January 1999

OSTI ID:872383

Nivens, David E ^[1]; Applegate, Bruce M ^[2]

11912 Kingsgate Rd., Knoxville, TN 37911
3700 Sutherland Ave. #Q2, Knoxville, TN 37911

A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.

View Patent

Research Organization:: Microbial Insight, Inc., Knoxville, TN (United States)

DOE Contract Number:: FG02-95ER82027

Assignee:: Nivens, David E. (11912 Kingsgate Rd., Knoxville, TN 37911);Applegate, Bruce M. (3700 Sutherland Ave. #Q2, Knoxville, TN 37911)

Patent Number(s):: US 5922536

OSTI ID:: 872383

Country of Publication:: United States

Language:: English

References (15)

DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community † Holben, William E.; Jansson, Janet K.; Chelm, Barry K. Applied and Environmental Microbiology, Vol. 54, Issue 3 https://doi.org/10.1128/AEM.54.3.703-711.1988	journal	January 1988
Membrane-bound DNA from Escherichia coli : Extraction by freeze-thaw-lysozyme Grossman, Nili; Ron, Eliora Z. FEBS Letters, Vol. 54, Issue 3 https://doi.org/10.1016/0014-5793(75)80932-X	journal	July 1975
Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction. Picard, C.; Ponsonnet, C.; Paget, E. Applied and Environmental Microbiology, Vol. 58, Issue 9 https://doi.org/10.1128/AEM.58.9.2717-2722.1992	journal	January 1992
Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid. Bej, A. K.; DiCesare, J. L.; Haff, L. Applied and Environmental Microbiology, Vol. 57, Issue 4 https://doi.org/10.1128/AEM.57.4.1013-1017.1991	journal	January 1991
Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Johnson, D. W.; Pieniazek, N. J.; Griffin, D. W. Applied and environmental microbiology, Vol. 61, Issue 11 https://doi.org/10.1128/AEM.61.11.3849-3855.1995	journal	January 1995
The extraction and purification of microbial DNA from sediments Ogram, Andrew; Sayler, Gary S.; Barkay, Tamar Journal of Microbiological Methods, Vol. 7, Issue 2-3 https://doi.org/10.1016/0167-7012(87)90025-X	journal	December 1987
PCR Detection of Polycyclic Aromatic Hydrocarbon-Degrading Mycobacteria Wang, Rong-Fu; Luneau, Allison; Cao, Wei-Wen Environmental Science & Technology, Vol. 30, Issue 1 https://doi.org/10.1021/es950388b	journal	January 1996
Quantitative cell lysis of indigenous microorganisms and rapid extraction of microbial DNA from sediment. Moré, M. I.; Herrick, J. B.; Silva, M. C. Applied and Environmental Microbiology, Vol. 60, Issue 5 https://doi.org/10.1128/AEM.60.5.1572-1580.1994	journal	January 1994
Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples. Bej, A. K.; Mahbubani, M. H.; Dicesare, J. L. Applied and Environmental Microbiology, Vol. 57, Issue 12 https://doi.org/10.1128/AEM.57.12.3529-3534.1991	journal	January 1991
Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction. Tsai, Y. L.; Olson, B. H. Applied and Environmental Microbiology, Vol. 58, Issue 7 https://doi.org/10.1128/AEM.58.7.2292-2295.1992	journal	January 1992
Rapid method for direct extraction of DNA from soil and sediments. Tsai, Y. L.; Olson, B. H. Applied and Environmental Microbiology, Vol. 57, Issue 4 https://doi.org/10.1128/AEM.57.4.1070-1074.1991	journal	January 1991
Simple, rapid method for direct isolation of nucleic acids from aquatic environments. Somerville, C. C.; Knight, I. T.; Straube, W. L. Applied and Environmental Microbiology, Vol. 55, Issue 3 https://doi.org/10.1128/AEM.55.3.548-554.1989	journal	January 1989
Recovery of DNA from soils and sediments. Steffan, R. J.; Goksøyr, J.; Bej, A. K. Applied and Environmental Microbiology, Vol. 54, Issue 12 https://doi.org/10.1128/AEM.54.12.2908-2915.1988	journal	January 1988
Detection of Escherichia coli in sewage and sludge by polymerase chain reaction. Tsai, Y. L.; Palmer, C. J.; Sangermano, L. R. Applied and Environmental Microbiology, Vol. 59, Issue 2 https://doi.org/10.1128/AEM.59.2.353-357.1993	journal	January 1993
Detection of coliform bacteria in water by polymerase chain reaction and gene probes. Bej, A. K.; Steffan, R. J.; DiCesare, J. Applied and Environmental Microbiology, Vol. 56, Issue 2 https://doi.org/10.1128/AEM.56.2.307-314.1990	journal	January 1990

Similar Records

Method for nucleic acid isolation using supercritical fluids

Patent · 1999 · OSTI ID:6377626

Method for isolating nucleic acids

Patent · 2015 · OSTI ID:1221979

Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe

Patent · 1999 · OSTI ID:872867

Related Subjects

/435/999/
acid
acid isolation
contacting
detecting
environmental
environmental sample
fluid
fluids
involves
involves contacting
isolate
isolated
isolation
method
microorganism
nucleic
nucleic acid
optionally
particular
presence
purification
sample
sequence
subjected
supercritical
supercritical fluid
supercritical fluids

Method for nucleic acid isolation using supercritical fluids

Citation Formats

References (15)

Similar Records

Related Subjects