Method for nucleic acid isolation using supercritical fluids

Nivens, David E; Applegate, Bruce M

Title: Method for nucleic acid isolation using supercritical fluids

Patent · Fri Jan 01 00:00:00 EST 1999

OSTI ID:872383

Nivens, David E ^[1]; Applegate, Bruce M ^[2]

11912 Kingsgate Rd., Knoxville, TN 37911
3700 Sutherland Ave. #Q2, Knoxville, TN 37911

A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.

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Research Organization:: Microbial Insight, Inc., Knoxville, TN (United States)

DOE Contract Number:: FG02-95ER82027

Assignee:: Nivens, David E. (11912 Kingsgate Rd., Knoxville, TN 37911);Applegate, Bruce M. (3700 Sutherland Ave. #Q2, Knoxville, TN 37911)

Patent Number(s):: US 5922536

OSTI ID:: 872383

Country of Publication:: United States

Language:: English

References (13)

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Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples. Bej, A. K.; Mahbubani, M. H.; Dicesare, J. L. Applied and Environmental Microbiology, Vol. 57, Issue 12 https://doi.org/10.1128/AEM.57.12.3529-3534.1991	journal	January 1991
Detection of Escherichia coli in sewage and sludge by polymerase chain reaction. Tsai, Y. L.; Palmer, C. J.; Sangermano, L. R. Applied and Environmental Microbiology, Vol. 59, Issue 2 https://doi.org/10.1128/AEM.59.2.353-357.1993	journal	January 1993
Recovery of DNA from soils and sediments. Steffan, R. J.; Goksøyr, J.; Bej, A. K. Applied and Environmental Microbiology, Vol. 54, Issue 12 https://doi.org/10.1128/AEM.54.12.2908-2915.1988	journal	January 1988
PCR Detection of Polycyclic Aromatic Hydrocarbon-Degrading Mycobacteria Wang, Rong-Fu; Luneau, Allison; Cao, Wei-Wen Environmental Science & Technology, Vol. 30, Issue 1 https://doi.org/10.1021/es950388b	journal	January 1996
Detection of coliform bacteria in water by polymerase chain reaction and gene probes. Bej, A. K.; Steffan, R. J.; DiCesare, J. Applied and Environmental Microbiology, Vol. 56, Issue 2 https://doi.org/10.1128/AEM.56.2.307-314.1990	journal	January 1990
Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Johnson, D. W.; Pieniazek, N. J.; Griffin, D. W. Applied and environmental microbiology, Vol. 61, Issue 11 https://doi.org/10.1128/AEM.61.11.3849-3855.1995	journal	January 1995
Simple, rapid method for direct isolation of nucleic acids from aquatic environments. Somerville, C. C.; Knight, I. T.; Straube, W. L. Applied and Environmental Microbiology, Vol. 55, Issue 3 https://doi.org/10.1128/AEM.55.3.548-554.1989	journal	January 1989
Quantitative cell lysis of indigenous microorganisms and rapid extraction of microbial DNA from sediment. Moré, M. I.; Herrick, J. B.; Silva, M. C. Applied and Environmental Microbiology, Vol. 60, Issue 5 https://doi.org/10.1128/AEM.60.5.1572-1580.1994	journal	January 1994
Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction. Tsai, Y. L.; Olson, B. H. Applied and Environmental Microbiology, Vol. 58, Issue 7 https://doi.org/10.1128/AEM.58.7.2292-2295.1992	journal	January 1992
Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid. Bej, A. K.; DiCesare, J. L.; Haff, L. Applied and Environmental Microbiology, Vol. 57, Issue 4 https://doi.org/10.1128/AEM.57.4.1013-1017.1991	journal	January 1991
The extraction and purification of microbial DNA from sediments Ogram, Andrew; Sayler, Gary S.; Barkay, Tamar Journal of Microbiological Methods, Vol. 7, Issue 2-3 https://doi.org/10.1016/0167-7012(87)90025-X	journal	December 1987

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method
nucleic
acid
isolation
supercritical
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detecting
presence
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contacting
fluid
isolate
particular
sequence
isolated
optionally
subjected
purification
involves contacting
environmental sample
supercritical fluid
nucleic acid
supercritical fluids
acid isolation
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Title: Method for nucleic acid isolation using supercritical fluids

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References (13)

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