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Title: cDNA encoding a polypeptide including a hevein sequence

Patent ·
OSTI ID:872273
 [1];  [2];  [3];  [4]
  1. Okemos, MI
  2. Dilbeek, BE
  3. Scarsdale, NY
  4. New York, NY
+ Show Author Affiliations

A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

Research Organization:
Michigan State Univ., East Lansing, MI (United States)
DOE Contract Number:
AC02-76ER01338
Assignee:
Board of Trustees operating Michigan State University (East Lansing, MI)
Patent Number(s):
US 5900480
Application Number:
07/888,366
OSTI ID:
872273
Country of Publication:
United States
Language:
English

References (6)

Isolation of cDNA clones coding for spinach nitrite reductase: Complete sequence and nitrate induction journal April 1988
Co- and post-translational processing of the hevein preproprotein of latex of the rubber tree (Hevea brasiliensis) journal August 1991
The gene for the precursor of plastocyanin from the cyanobacterium Anabaena sp. PCC 7937: isolation, sequence and regulation journal March 1989
A homologue of the nuclear coded 49 kd subunit of bovine mitochondrial NADH-ubiquinone reductase is coded in chloroplast DNA. journal March 1989
Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis). journal October 1990
Plastid-localised seed acyl-carrier protein of Brassica napus is encoded by a distinct, nuclear multigene family journal June 1988

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cdna
encoding
polypeptide
including
hevein
sequence
clone
hev1
isolated
via
polymerase
chain
reaction
pcr
mixed
oligonucleotides
corresponding
regions
primers
hevea
brasiliensis
latex
library
template
1018
nucleotides
reading
frame
204
amino
acids
deduced
acid
contains
putative
signal
17
residues
followed
187
amino-terminal
region
43
identical
homology
chitin-binding
proteins
amino-termini
wound-induced
genes
potato
poplar
carboxyl-terminal
portion
144
74-79
homologous
wound-inducible
wounding
application
plant
hormones
abscisic
ethylene
resulted
accumulation
transcripts
leaves
stems
roots
shown
probe
fusion
protein
produced
coli
maltose
binding
cdna clone
deduced amino
reading frame
chain reaction
binding protein
acid sequence
amino acid
polymerase chain
amino acids
cdna library
dna encoding
cdna encoding
residues followed
protein produced
putative signal
wound-induced genes
wound-inducible genes
abscisic acid
carboxyl-terminal portion
carboxyl-terminal region
maltose binding
plant hormones
chitin-binding proteins
amino-terminal region
sequence contains
signal sequence
oligonucleotides corresponding
mixed oligonucleotides
via polymerase
encoding hevein
hevea brasiliensis
acid residues
acid residue
acid polypeptide
brasiliensis latex
latex cdna
isolated via
polypeptide including
fusion protein
hormones abscisic
hevein sequence
hevein transcripts
nucleotides corresponding
/999/435/