CDNA encoding a polypeptide including a hevein sequence

Raikhel, Natasha V; Broekaert, Willem F; Chua, Nam-Hai; Kush, Anil

CDNA encoding a polypeptide including a hevein sequence

Patent · Mon Mar 20 23:00:00 EST 1995

OSTI ID:869795

Raikhel, Natasha V ^[1]; Broekaert, Willem F ^[2]; Chua, Nam-Hai ^[3]; Kush, Anil ^[4]

Okemos, MI
Dilbeek, BE
Scarsdale, NY
New York, NY

A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

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Research Organization:: MICHIGAN STATE UNIVERSITY

DOE Contract Number:: AC02-76ER01338

Assignee:: Board of Trustees operating Michigan State University (East Lansing, MI)

Patent Number(s):: US 5399668

Application Number:: 07/888,364

OSTI ID:: 869795

Country of Publication:: United States

Language:: English

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/530/435/999/
1018
144
17
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204
43
74-79
abscisic
abscisic acid
accumulation
acid
acid polypeptide
acid residue
acid residues
acid sequence
acids
amino
amino acid
amino acids
amino-terminal
amino-terminal region
amino-termini
application
binding
binding protein
brasiliensis
brasiliensis latex
carboxyl-terminal
carboxyl-terminal portion
carboxyl-terminal region
cdna
cdna clone
cdna encoding
cdna library
chain
chain reaction
chitin-binding
chitin-binding proteins
clone
coli
contains
corresponding
deduced
deduced amino
dna encoding
encoding
encoding hevein
ethylene
followed
frame
fusion
fusion protein
genes
hev1
hevea
hevea brasiliensis
hevein
hevein sequence
hevein transcripts
homologous
homology
hormones
hormones abscisic
identical
including
isolated
isolated via
latex
latex cdna
leaves
library
maltose
maltose binding
mixed
mixed oligonucleotides
nucleotides
nucleotides corresponding
oligonucleotides
oligonucleotides corresponding
pcr
plant
plant hormones
polymerase
polymerase chain
polypeptide
polypeptide including
poplar
portion
potato
primers
probe
produced
protein
protein produced
proteins
putative
putative signal
reaction
reading
reading frame
region
regions
residues
residues followed
resulted
roots
sequence
sequence contains
shown
signal
signal sequence
stems
template
transcripts
via
via polymerase
wound-induced
wound-induced genes
wound-inducible
wound-inducible genes
wounding

CDNA encoding a polypeptide including a hevein sequence

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