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Title: Method for identifying and quantifying nucleic acid sequence aberrations

Patent ·
OSTI ID:871727
 [1];  [2];  [3]
  1. San Ramon, CA
  2. Tracy, CA
  3. Walnut Creek, CA
+ Show Author Affiliations

A method for detecting nucleic acid sequence aberrations by detecting nucleic acid sequences having both a first and a second nucleic acid sequence type, the presence of the first and second sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. The method uses a first hybridization probe which includes a nucleic acid sequence that is complementary to a first sequence type and a first complexing agent capable of attaching to a second complexing agent and a second hybridization probe which includes a nucleic acid sequence that selectively hybridizes to the second nucleic acid sequence type over the first sequence type and includes a detectable marker for detecting the second hybridization probe.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
DOE Contract Number:
W-7405-ENG-48
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 5783387
OSTI ID:
871727
Country of Publication:
United States
Language:
English

References (15)

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A versatile acid-labile linker for modification of synthetic biomolecules journal January 1990
A cleavable biotinylating agent and its use in protein electroblots journal August 1992
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Analytical strategies for the use of DNA probes journal February 1988
A ligase-mediated gene detection technique journal August 1988
A novel method to map transcripts: Evidence for homology between an adenovirus mRNA and discrete multiple regions of the viral genome journal September 1977
Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. journal December 1988
Two-step affinity chromatography. Model systems and an example using biotin-avidin binding and a fluoridolyzable linker journal November 1991
Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization journal October 1990
Affinity purification method using a reversible biotinylating reagent for peptides synthesized by the solid-phase technique journal May 1993
Facile Preparation and some Applications of an Affinity Matrix with a Cleavable Connector arm Containing a Disulfide bond journal June 1987
Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. journal May 1986
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. journal July 1983

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Related Subjects

method
identifying
quantifying
nucleic
acid
sequence
aberrations
detecting
sequences
type
presence
indicating
aberration
hybridization
probe
complementary
complexing
agent
capable
attaching
selectively
hybridizes
detectable
marker
sequence aberration
detecting nucleic
detectable marker
sequence aberrations
sequence type
acid sequence
nucleic acid
acid sequences
complexing agent
hybridization probe
agent capable
sequence indicating
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