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Method for identifying and quantifying nucleic acid sequence aberrations

Patent ·
OSTI ID:871727
 [1];  [2];  [3]
  1. San Ramon, CA
  2. Tracy, CA
  3. Walnut Creek, CA
+ Show Author Affiliations
A method for detecting nucleic acid sequence aberrations by detecting nucleic acid sequences having both a first and a second nucleic acid sequence type, the presence of the first and second sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. The method uses a first hybridization probe which includes a nucleic acid sequence that is complementary to a first sequence type and a first complexing agent capable of attaching to a second complexing agent and a second hybridization probe which includes a nucleic acid sequence that selectively hybridizes to the second nucleic acid sequence type over the first sequence type and includes a detectable marker for detecting the second hybridization probe.
Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
DOE Contract Number:
W-7405-ENG-48
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 5783387
OSTI ID:
871727
Country of Publication:
United States
Language:
English

References (15)

A cleavable biotinylating agent and its use in protein electroblots journal August 1992
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. journal July 1983
Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. journal May 1986
A rapid method of gene detection using DNA bound to Sephacryl journal January 1985
A novel method to map transcripts: Evidence for homology between an adenovirus mRNA and discrete multiple regions of the viral genome journal September 1977
Analytical strategies for the use of DNA probes journal February 1988
Facile Preparation and some Applications of an Affinity Matrix with a Cleavable Connector arm Containing a Disulfide bond journal June 1987
Affinity purification method using a reversible biotinylating reagent for peptides synthesized by the solid-phase technique journal May 1993
A versatile acid-labile linker for modification of synthetic biomolecules journal January 1990
Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. journal December 1988
Two-step affinity chromatography. Model systems and an example using biotin-avidin binding and a fluoridolyzable linker journal November 1991
Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization journal October 1990
A ligase-mediated gene detection technique journal August 1988
Rapid Translocation Frequency Analysis in Humans Decades after Exposure to Ionizing Radiation journal January 1992
A reagent for covalently attaching biotin to proteins via a cleavable connector arm journal October 1982

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Related Subjects

/435/999/
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