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Title: Cytoplasmic bacteriophage display system

Patent ·
OSTI ID:871626
 [1];  [2]
  1. Stony Brook, NY
  2. Setauket, NY
+ Show Author Affiliations

Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest.

Research Organization:
Associated Universities, Inc., Upton, NY (United States)
DOE Contract Number:
AC02-76CH00016
Assignee:
Associated Universities Inc. (Washington, DC)
Patent Number(s):
US 5766905
Application Number:
08/664,449
OSTI ID:
871626
Country of Publication:
United States
Language:
English

References (8)

Peptides on phage: a vast library of peptides for identifying ligands journal August 1990
Antibody-selectable filamentous fd phage vectors: affinity purification of target genes journal December 1988
Lambda foo: a lambda phage vector for the expression of foreign proteins. journal August 1994
Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface journal June 1985
Searching for peptide ligands with an epitope library journal July 1990
Expression of human factor IX and its subfragments in Escherichia coli and generation of antibodies to the subfragments journal August 1987
Phage display: protein engineering by directed evolution journal August 1995
Display of peptides and proteins on the surface of bacteriophage lambda. journal February 1995

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cytoplasmic
bacteriophage
display
disclosed
vectors
comprising
dna
encoding
portion
structural
protein
joined
covalently
peptide
exemplified
t7
capsid
specifically
c-terminal
amino
acid
residue
n-terminal
comprises
corresponding
form
10b
useful
copy
larger
fusion
compositions
type
described
connection
methods
producing
virus
displaying
amino acid
dna encoding
type described
display vectors
acid residue
structural protein
cytoplasmic bacteriophage
/435/424/