skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cytoplasmic bacteriophage display system

Abstract

Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest. 1 fig.

Inventors:
;
Publication Date:
Research Org.:
Associated Universities Inc
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
672569
Patent Number(s):
US 5,766,905/A/
Application Number:
PAN: 8-664,449
Assignee:
Associated Universities Inc., Washington, DC (United States) PTO; SCA: 550700; 550200; 550900; PA: EDB-98:122147; SN: 98002024901
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 16 Jun 1998
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; BACTERIOPHAGES; DNA; CYTOPLASM; PROTEINS; VIRUSES

Citation Formats

Studier, F.W., and Rosenberg, A.H. Cytoplasmic bacteriophage display system. United States: N. p., 1998. Web.
Studier, F.W., & Rosenberg, A.H. Cytoplasmic bacteriophage display system. United States.
Studier, F.W., and Rosenberg, A.H. Tue . "Cytoplasmic bacteriophage display system". United States.
@article{osti_672569,
title = {Cytoplasmic bacteriophage display system},
author = {Studier, F.W. and Rosenberg, A.H.},
abstractNote = {Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest. 1 fig.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 16 00:00:00 EDT 1998},
month = {Tue Jun 16 00:00:00 EDT 1998}
}