Cytoplasmic bacteriophage display system

Patent · Tue Jun 16 04:00:00 EDT 1998

OSTI ID:672569

Studier, F W; Rosenberg, A H

Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest. 1 fig.

• Research Organization: Associated Universities Inc
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC02-76CH00016
• Assignee: Associated Universities Inc., Washington, DC (United States)
• Patent Number(s): US 5,766,905/A/
• Application Number: PAN: 8-664,449
• OSTI ID: 672569
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BACTERIOPHAGES
CYTOPLASM
DNA
PROTEINS
VIRUSES