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Modified acyl-ACP desaturase

Patent ·
OSTI ID:871309
 [1];  [1];  [2];  [2]
  1. Shoreham, NY
  2. Jarfalla, SE
+ Show Author Affiliations

Disclosed is a methods for modifying the chain length and double bond positional specificities of a soluble plant fatty acid desaturase. More specifically, the method involves modifying amino acid contact residues in the substrate binding channel of the soluble fatty acid desaturase which contact the fatty acid. Specifically disclosed is the modification of an acyl-ACP desaturase. Amino acid contact residues which lie within the substrate binding channel are identified, and subsequently replaced with different residues to effect the modification of activity.

Research Organization:
ASSOC UNIVERSITIES INC
DOE Contract Number:
AC02-76CH00016
Assignee:
Associated Universities, Inc. (Washington, DC)
Patent Number(s):
US 5705391
Application Number:
08/689,823
OSTI ID:
871309
Country of Publication:
United States
Language:
English

References (7)

delta 6 Hexadecenoic acid is synthesized by the activity of a soluble delta 6 palmitoyl-acyl carrier protein desaturase in Thunbergia alata endosperm. journal November 1994
Di-iron—carboxylate proteins journal December 1995
Crystal structure of a bacterial non-haem iron hydroxylase that catalyses the biological oxidation of methane journal December 1993
Stearoyl-acyl carrier protein delta 9 desaturase from Ricinus communis is a diiron-oxo protein. journal March 1993
Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco. journal December 1992
Structure and Function of the Escherichia coli Ribonucleotide Reductase Protein R2 journal July 1993
Eight Histidine Residues Are Catalytically Essential in a Membrane-Associated Iron Enzyme, Stearoyl-CoA Desaturase, and Are Conserved in Alkane Hydroxylase and Xylene Monooxygenase journal January 1994

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Related Subjects

/435/999/
acid
acid contact
acid desaturase
activity
acyl-acp
acyl-acp desaturase
amino
amino acid
binding
bond
bond positional
chain
chain length
channel
contact
contact residues
desaturase
disclosed
double
double bond
effect
fatty
fatty acid
identified
involves
involves modifying
length
lie
method
method involve
method involves
methods
modification
modified
modified acyl-acp
modifying
modifying amino
plant
plant fatty
positional
positional specificities
replaced
residues
soluble
soluble plant
specifically
specifically disclosed
specificities
subsequently
substrate