Procedure for normalization of cDNA libraries
- New York, NY
This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.
- Research Organization:
- Columbia University
- DOE Contract Number:
- FG02-91ER61233
- Assignee:
- Trustees of Columbia University in City of New York (New York, NY)
- Patent Number(s):
- US 5702898
- OSTI ID:
- 871292
- Country of Publication:
- United States
- Language:
- English
Molecular cloning and nucleotide sequence of cDNA for human liver arginase.
|
journal | January 1987 |
Construction of a uniform-abundance (normalized) cDNA library.
|
journal | March 1991 |
Similar Records
Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
Related Subjects
acid
acid molecule
acid molecules
appropriate
appropriate cot
capable
cdna
cdna libraries
cdna library
circles
complementary
complementary nucleic
comprising
constructed
converted
converting
cot
duplexes
generating
hybridized
hybridizing
libraries
library
library constructed
method
molecules
normalization
normalize
normalized
normalized cdna
nucleic
nucleic acid
partial
partial duplexes
procedure
produce
produce partial
producing
provides
separating
single-stranded
single-stranded circle
single-stranded circles
step
unhybridized
vector