Procedure for normalization of cDNA libraries
This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library. 1 fig.
- Research Organization:
- Columbia Univ., New York, NY (United States)
- Sponsoring Organization:
- National Insts. of Health, Bethesda, MD (United States); Department of Health and Human Services, Washington, DC (United States); USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-91ER61233
- Assignee:
- Columbia Univ., New York, NY (United States)
- Patent Number(s):
- US 5,702,898/A/
- Application Number:
- PAN: 8-465,857; CNN: Grant R01-HG00980
- OSTI ID:
- 563674
- Resource Relation:
- Other Information: PBD: 30 Dec 1997
- Country of Publication:
- United States
- Language:
- English
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