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Title: Cloning and expression of the gene for bacteriophage T7 RNA polymerase

Patent ·
OSTI ID:871257
 [1];  [2];  [3];  [4];  [5]
  1. Stony Brook, NY
  2. Basel, CH
  3. Setauket, NY
  4. Waterloo, CA
  5. Bellport, NY
+ Show Author Affiliations

This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.

Research Organization:
Associated Universities, Inc., Upton, NY (United States)
DOE Contract Number:
AC02-76CH00016
Assignee:
Associated Universities, Inc. (Washington, DC)
Patent Number(s):
US 5693489
Application Number:
08/259,560
OSTI ID:
871257
Country of Publication:
United States
Language:
English

References (6)

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. journal February 1985
Organization and Expression of Bacteriophage T7 DNA journal January 1983
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements journal June 1983
T7 RNA polymerase directed expression of the Escherichia coli rrnB operon. journal May 1986
Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase journal February 1984
A bacteriophage lambda vector for cloning with BamHI and Sau3A journal December 1982

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