Cloning and expression of the gene for bacteriophage T7 RNA polymerase
This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells. 10 figs.
- Research Organization:
- Associated Universities Inc
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC02-76CH00016
- Assignee:
- Associated Universities, Inc., Washington, DC (United States)
- Patent Number(s):
- US 5,693,489/A/
- Application Number:
- PAN: 8-259,560
- OSTI ID:
- 563682
- Country of Publication:
- United States
- Language:
- English
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Cloning and expression of the gene for bacteriophage T7 RNA polymerase
Cloning and expression of the gene for bacteriophage T7 RNA polymerase