Large scale DNA microsequencing device

Patent · Wed Jan 01 00:00:00 EST 1997

OSTI ID:871114

Foote, Robert S ^[1]

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1. Oak Ridge, TN

A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
• DOE Contract Number: AC05-84OR21400
• Assignee: Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN)
• Patent Number(s): US 5661028
• OSTI ID: 871114
• Country of Publication: United States
• Language: English

Related Subjects

scale
dna
microsequencing
device
microminiature
sequencing
apparatus
method
provide
means
simultaneously
obtaining
sequences
plural
polynucleotide
strands
comprises
microchip
channels
etched
standard
lithographic
procedures
chemical
wet
etching
reaction
separating
section
enclosing
accomplished
bonding
transparent
cover
plate
oligonucleotide
strand
chemically
affixed
alkyl
chain
subsequent
nucleotides
selected
complementary
base
pair
target
nucleotide
produce
family
labelled
channel
separated
following
separation
determined
appropriate
detection
oligonucleotide strand
alkyl chain
chemical wet
transparent cover
detection means
apparatus comprises
cover plate
method provide
wet etching
provide means
target nucleotide
apparatus comprise
complementary base
sequencing apparatus
base pair
scale dna
simultaneously obtaining
nucleotide strand
nucleotide strands
microsequencing device
microminiature sequencing
obtaining sequences
polynucleotide strands
plural polynucleotide
plural channels
dna microsequencing
lithographic procedures
/435/204/216/423/999/