Large scale DNA microsequencing device

Patent · 31 December 1998

OSTI ID:872484

Foote, Robert S ^[1]

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1. Oak Ridge, TN

A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.

• Research Organization: LOCKHEED MARTIN ENRGY SYST INC
• DOE Contract Number: AC05-84OR21400
• Assignee: Lockheed Martin Energy Research Corporation (Oak Ridge, TN)
• Patent Number(s): US 5944971
• OSTI ID: 872484
• Country of Publication: United States
• Language: English

Related Subjects

/204/435/
accomplished
affixed
alkyl
alkyl chain
apparatus
apparatus comprise
apparatus comprises
appropriate
base
base pair
bonding
chain
channel
channels
chemical
chemical wet
chemically
complementary
complementary base
comprises
cover
cover plate
detection
detection means
determined
device
dna
dna microsequencing
enclosing
etched
etching
family
following
labelled
lithographic
lithographic procedures
means
method
method provide
microchip
microminiature
microminiature sequencing
microsequencing
microsequencing device
nucleotide
nucleotide strand
nucleotide strands
nucleotides
obtaining
obtaining sequences
oligonucleotide
oligonucleotide strand
pair
plate
plural
plural channels
plural polynucleotide
polynucleotide
polynucleotide strands
procedures
produce
provide
provide means
reaction
scale
scale dna
section
selected
separated
separating
separation
sequences
sequencing
sequencing apparatus
simultaneously
simultaneously obtaining
standard
strand
strands
subsequent
target
target nucleotide
transparent
transparent cover
wet
wet etching