Method to transform algae, materials therefor, and products produced thereby

Dunahay, Terri Goodman; Roessler, Paul G; Jarvis, Eric E

Title: Method to transform algae, materials therefor, and products produced thereby

Patent · Wed Jan 01 00:00:00 EST 1997

OSTI ID:871113

Dunahay, Terri Goodman ^[1]; Roessler, Paul G ^[2]; Jarvis, Eric E ^[3]

2710 Arbor Glen Pl., Boulder, CO 80304
15905 Ellsworth Pl., Golden, CO 80401
3720 Smuggler Pl., Boulder, CO 80303

Disclosed is a method to transform chlorophyll C-containing algae which includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae.

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Research Organization:: Midwest Research Institute, Kansas City, MO (United States)

DOE Contract Number:: AC36-83CH10093

Assignee:: Dunahay, Terri Goodman (2710 Arbor Glen Pl., Boulder, CO 80304);Roessler, Paul G. (15905 Ellsworth Pl., Golden, CO 80401);Jarvis, Eric E. (3720 Smuggler Pl., Boulder, CO 80303)

Patent Number(s):: US 5661017

Application Number:: 08/404732

OSTI ID:: 871113

Country of Publication:: United States

Language:: English

References (19)

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method
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algae
materials
products
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chlorophyll
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introducing
recombinant
molecule
comprising
nucleic
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encoding
dominant
selectable
marker
operatively
linked
algal
regulatory
control
sequence
alga
manner
preferred
embodiment
derived
diatom
preferably
cyclotella
cryptica
chimeric
sequences
rna
protein
normally
occur
specifically
molecules
paccnpt10
paccnpt4
paccnpt5
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ability
accomplish
stable
genetic
transformation
acid molecule
specifically disclosed
nucleic acid
preferred embodiment
products produced
operatively linked
molecule comprising
cyclotella cryptica
control sequences
normally occur
recombinant molecule
control sequence
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Title: Method to transform algae, materials therefor, and products produced thereby

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References (19)

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