Luminescent lanthanide chelates and methods of use
- Berkeley, CA
The invention provides lanthanide chelates capable of intense luminescence. The celates comprise a lanthanide chelator covalently joined to a coumarin-like or quinolone-like sensitizer. Exemplary sensitzers include 2- or 4-quinolones, 2- or 4-coumarins, or derivatives thereof e.g. carbostyril 124 (7-amino-4-methyl-2-quinolone), coumarin 120 (7-amino-4-methyl-2-coumarin), coumarin 124 (7-amino-4-(trifluoromethyl)-2-coumarin), aminomethyltrimethylpsoralen, etc. The chelates form high affinity complexes with lanthanides, such as terbium or europium, through chelator groups, such as DTPA. The chelates may be coupled to a wide variety of compounds to create specific labels, probes, diagnostic and/or therapeutic reagents, etc. The chelates find particular use in resonance energy transfer between chelate-lanthanide complexes and another luminescent agent, often a fluorescent non-metal based resonance energy acceptor. The methods provide useful information about the structure, conformation, relative location and/or interactions of macromolecules.
- Research Organization:
- Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Number(s):
- US 5639615
- OSTI ID:
- 871004
- Country of Publication:
- United States
- Language:
- English
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/435/436/
120
124
2-
4-coumarins
4-quinolones
7-amino-4-
7-amino-4-methyl-2-coumarin
7-amino-4-methyl-2-quinolone
acceptor
affinity
agent
aminomethyltrimethylpsoralen
based
capable
carbostyril
celates
celates comprise
chelate-lanthanide
chelates
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chelates form
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comprise
conformation
coumarin
coumarin-like
coupled
covalently
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diagnostic
dtpa
energy
energy transfer
etc
europium
exemplary
fluorescent
form
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intense
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interactions
joined
labels
lanthanide
lanthanide chelates
lanthanide chelator
lanthanides
location
luminescence
luminescent
luminescent lanthanide
macromolecules
metal base
metal based
methods
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non-metal
particular
probes
provide
provide useful
provides
provides lanthanide
quinolone-like
quinolone-like sensitizer
reagents
relative
relative location
resonance
resonance energy
sensitizer
sensitzers
specific
structure
terbium
therapeutic
transfer
trifluoromethyl
useful
useful information
variety
wide
wide variety