Luminescent lanthanide chelates and methods of use

Selvin, Paul R; Hearst, John

Luminescent lanthanide chelates and methods of use

Patent · Tue Dec 31 23:00:00 EST 1996

OSTI ID:870909

Selvin, Paul R ^[1]; Hearst, John ^[1]

Berkeley, CA

The invention provides lanthanide chelates capable of intense luminescence. The celates comprise a lanthanide chelator covalently joined to a coumarin-like or quinolone-like sensitizer. Exemplary sensitzers include 2- or 4-quinolones, 2- or 4-coumarins, or derivatives thereof e.g. carbostyril 124 (7-amino-4-methyl-2-quinolone), coumarin 120 (7-amino-4-methyl-2-coumarin), coumarin 124 (7-amino-4-(trifluoromethyl)-2-coumarin), aminomethyltrimethylpsoralen, etc. The chelates form high affinity complexes with lanthanides, such as terbium or europium, through chelator groups, such as DTPA. The chelates may be coupled to a wide variety of compounds to create specific labels, probes, diagnostic and/or therapeutic reagents, etc. The chelates find particular use in resonance energy transfer between chelate-lanthanide complexes and another luminescent agent, often a fluorescent non-metal based resonance energy acceptor. The methods provide useful information about the structure, conformation, relative location and/or interactions of macromolecules.

Research Organization:: Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA

Assignee:: Regents of University of California (Oakland, CA)

Patent Number(s):: US 5622821

OSTI ID:: 870909

Country of Publication:: United States

Language:: English

References (11)

[13] Fluorescence resonance energy transfer Selvin, Paul R. Methods in Enzymology https://doi.org/10.1016/0076-6879(95)46015-2	book	January 1995
Rare earth cryptates and homogeneous fluoroimmunoassays with human sera Mathis, G. Clinical Chemistry, Vol. 39, Issue 9 https://doi.org/10.1093/clinchem/39.9.1953	journal	September 1993
Europium and terbium chelators as candidate substrates for enzyme-labelled time-resolved fluorimetric immunoassays Diamandis, Eleftherios P. The Analyst, Vol. 117, Issue 12 https://doi.org/10.1039/an9921701879	journal	January 1992
Time-resolved fluorescence imaging of europium chelate label in immunohistochemistry and in situ hybridization Seveus, Lahja; Väisälä, Mikko; Syrjänen, Stina Cytometry, Vol. 13, Issue 4 https://doi.org/10.1002/cyto.990130402	journal	January 1992
Time-resolved fluorescence of a new europium-chelate complex: demonstration of highly sensitive detection of protein and DNA samples Saha, Ashis K.; Kross, Kathryn; Kloszewski, Edmond D. Journal of the American Chemical Society, Vol. 115, Issue 23, p. 11032-11033 https://doi.org/10.1021/ja00076a088	journal	November 1993
Fluorescent terbium chelates derived from diethylenetriaminepentaacetic acid and heterocyclic compounds Canfi, Ayala; Bailey, M. Philip; Rocks, Bernard F. The Analyst, Vol. 114, Issue 11 https://doi.org/10.1039/an9891401405	journal	January 1989
Study of europium-sensitized fluorescence of tetracycline in a micellar solution of Triton X-100 by fluorescence and thermal lens spectrometry Georges, J.; Ghazarian, S. Analytica Chimica Acta, Vol. 276, Issue 2 https://doi.org/10.1016/0003-2670(93)80411-D	journal	April 1993
Synthesis of a highly luminescent terbium chelate and its application to actin Ando, Toshio; Yamamoto, Tetsuya; Kobayashi, Nobuhiro Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1102, Issue 2 https://doi.org/10.1016/0005-2728(92)90099-N	journal	September 1992
Time-resolved fluorimetric detection of terbium-labelled deoxyribonucleic acid separated by gel electrophoresis Saavedra, Steven S.; Picozza, Enrico G. The Analyst, Vol. 114, Issue 7 https://doi.org/10.1039/an9891400835	journal	January 1989
Terbium chelate for use as a label in fluorescent immunoassays Bailey, Michael P.; Rocks, Bernard F.; Riley, Clifford The Analyst, Vol. 109, Issue 11 https://doi.org/10.1039/an9840901449	journal	January 1984
A Study of Sensitized Lanthanide Luminescence in an Engineered Calcium-Binding Protein Clark, I. D.; Macmanus, J. P.; Banville, D. Analytical Biochemistry, Vol. 210, Issue 1 https://doi.org/10.1006/abio.1993.1142	journal	April 1993

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Luminescent lanthanide chelates and methods of use

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