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Title: Normalized cDNA libraries

Abstract

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

Inventors:
 [1];  [2]
  1. (New York, NY)
  2. (Englewood, NJ)
Publication Date:
Research Org.:
Columbia University
OSTI Identifier:
870993
Patent Number(s):
US 5637685
Application Number:
08/465811
Assignee:
Trustees of Columbia University in City of New York (New York, NY) CHO
DOE Contract Number:  
FG02-91ER61233
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
normalized; cdna; libraries; provides; method; normalize; directional; library; constructed; vector; allows; propagation; single-stranded; circle; form; comprising; propagating; circles; generating; fragments; complementary; noncoding; sequence; produce; partial; duplexes; purifying; melting; reassociating; purified; moderate; cot; unassociated; cdna libraries; produce partial; directional cdna; single-stranded circles; cdna library; partial duplexes; normalized cdna; purified partial; single-stranded circle; unassociated single-stranded; noncoding sequence; circle form; allows propagation; coding sequence; library constructed; form comprising; generating fragments; fragments complementary; /999/435/

Citation Formats

Soares, Marcelo B., and Efstratiadis, Argiris. Normalized cDNA libraries. United States: N. p., 1997. Web.
Soares, Marcelo B., & Efstratiadis, Argiris. Normalized cDNA libraries. United States.
Soares, Marcelo B., and Efstratiadis, Argiris. Wed . "Normalized cDNA libraries". United States. doi:. https://www.osti.gov/servlets/purl/870993.
@article{osti_870993,
title = {Normalized cDNA libraries},
author = {Soares, Marcelo B. and Efstratiadis, Argiris},
abstractNote = {This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1997},
month = {Wed Jan 01 00:00:00 EST 1997}
}

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