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Title: Apparatus for improved DNA sequencing

Abstract

This invention is a means for the rapid sequencing of DNA samples. More specifically, it consists of a new design direct blotting electrophoresis unit. The DNA sequence is deposited on a membrane attached to a rotating drum. Initial data compaction is facilitated by the use of a machined multi-channeled plate called a ribbon channel plate. Each channel is an isolated mini gel system much like a gel filled capillary. The system as a whole, however, is in a slab gel like format with the advantages of uniformity and easy reusability. The system can be used in different embodiments. The drum system is unique in that after deposition the drum rotates the deposited DNA into a large non-buffer open space where processing and detection can occur. The drum can also be removed in toto to special workstations for downstream processing, multiplexing and detection.

Inventors:
 [1];  [2]
  1. Richland, WA
  2. Eltopia, WA
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA
OSTI Identifier:
870402
Patent Number(s):
US 5514256
Assignee:
Battelle Memorial Institute (Richland, WA)
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
apparatus; improved; dna; sequencing; means; rapid; samples; specifically; consists; design; direct; blotting; electrophoresis; unit; sequence; deposited; membrane; attached; rotating; drum; initial; data; compaction; facilitated; machined; multi-channeled; plate; called; ribbon; channel; isolated; mini; filled; capillary; slab; format; advantages; uniformity; easy; reusability; embodiments; unique; deposition; rotates; non-buffer; space; processing; detection; occur; removed; toto; special; workstations; downstream; multiplexing; channel plate; dna sequence; dna sequencing; rotating drum; initial data; data compaction; /204/

Citation Formats

Douthart, Richard J, and Crowell, Shannon L. Apparatus for improved DNA sequencing. United States: N. p., 1996. Web.
Douthart, Richard J, & Crowell, Shannon L. Apparatus for improved DNA sequencing. United States.
Douthart, Richard J, and Crowell, Shannon L. Mon . "Apparatus for improved DNA sequencing". United States. https://www.osti.gov/servlets/purl/870402.
@article{osti_870402,
title = {Apparatus for improved DNA sequencing},
author = {Douthart, Richard J and Crowell, Shannon L},
abstractNote = {This invention is a means for the rapid sequencing of DNA samples. More specifically, it consists of a new design direct blotting electrophoresis unit. The DNA sequence is deposited on a membrane attached to a rotating drum. Initial data compaction is facilitated by the use of a machined multi-channeled plate called a ribbon channel plate. Each channel is an isolated mini gel system much like a gel filled capillary. The system as a whole, however, is in a slab gel like format with the advantages of uniformity and easy reusability. The system can be used in different embodiments. The drum system is unique in that after deposition the drum rotates the deposited DNA into a large non-buffer open space where processing and detection can occur. The drum can also be removed in toto to special workstations for downstream processing, multiplexing and detection.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}

Patent:

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