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Apparatus for improved DNA sequencing

Patent ·
OSTI ID:870402
 [1];  [2]
  1. Richland, WA
  2. Eltopia, WA
+ Show Author Affiliations

This invention is a means for the rapid sequencing of DNA samples. More specifically, it consists of a new design direct blotting electrophoresis unit. The DNA sequence is deposited on a membrane attached to a rotating drum. Initial data compaction is facilitated by the use of a machined multi-channeled plate called a ribbon channel plate. Each channel is an isolated mini gel system much like a gel filled capillary. The system as a whole, however, is in a slab gel like format with the advantages of uniformity and easy reusability. The system can be used in different embodiments. The drum system is unique in that after deposition the drum rotates the deposited DNA into a large non-buffer open space where processing and detection can occur. The drum can also be removed in toto to special workstations for downstream processing, multiplexing and detection.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Number(s):
US 5514256
OSTI ID:
870402
Country of Publication:
United States
Language:
English

References (8)

High speed on-line DNA sequencing on ultrathin slab gels journal January 1991
Rapid DNA sequencing by horizontal ultrathin gel electrophoresis journal August 1991
Capillary gel electrophoresis for rapid, high resolution DNA sequencing journal January 1990
Applications of dioxetane chemiluminescent probes to molecular biology journal November 1990
Multiplex DNA sequencing journal April 1988
High speed DNA sequencing by capillary electrophoresis journal August 1990
[18] Direct transfer electrophoresis used for DNA sequencing book January 1987
Ultrathin-layer isoelectric focusing in 50-100 μm polyacrylamide gels on silanized glass plates or polyester films journal April 1980

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Related Subjects

/204/
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ribbon
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workstations