Apparatus for improved DNA sequencing
- Richland, WA
- Eltopia, WA
This invention is a means for the rapid sequencing of DNA samples. More specifically, it consists of a new design direct blotting electrophoresis unit. The DNA sequence is deposited on a membrane attached to a rotating drum. Initial data compaction is facilitated by the use of a machined multi-channeled plate called a ribbon channel plate. Each channel is an isolated mini gel system much like a gel filled capillary. The system as a whole, however, is in a slab gel like format with the advantages of uniformity and easy reusability. The system can be used in different embodiments. The drum system is unique in that after deposition the drum rotates the deposited DNA into a large non-buffer open space where processing and detection can occur. The drum can also be removed in toto to special workstations for downstream processing, multiplexing and detection.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- DOE Contract Number:
- AC06-76RL01830
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Number(s):
- US 5514256
- OSTI ID:
- 870402
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
improved
dna
sequencing
means
rapid
samples
specifically
consists
design
direct
blotting
electrophoresis
unit
sequence
deposited
membrane
attached
rotating
drum
initial
data
compaction
facilitated
machined
multi-channeled
plate
called
ribbon
channel
isolated
mini
filled
capillary
slab
format
advantages
uniformity
easy
reusability
embodiments
unique
deposition
rotates
non-buffer
space
processing
detection
occur
removed
toto
special
workstations
downstream
multiplexing
channel plate
dna sequence
dna sequencing
rotating drum
initial data
data compaction
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