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Method using CO for extending the useful shelf-life of refrigerated red blood cells

Patent ·
OSTI ID:870210
 [1]
  1. Los Alamos, NM
+ Show Author Affiliations
Method using CO for extending the useful shelf-life of refrigerated red blood cells. Carbon monoxide is utilized for stabilizing hemoglobin in red blood cells to be stored at low temperature. Changes observed in the stored cells are similar to those found in normal red cell aging in the body, the extent thereof being directly related to the duration of refrigerated storage. Changes in cell buoyant density, vesiculation, and the tendency of stored cells to bind autologous IgG antibody directed against polymerized band 3 IgG, all of which are related to red blood cell senescence and increase with refrigerated storage time, have been substantially slowed when red blood cells are treated with CO. Removal of the carbon monoxide from the red blood cells is readily and efficiently accomplished by photolysis in the presence of oxygen so that the stored red blood cells may be safely transfused into a recipient.
Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM
DOE Contract Number:
W-7405-ENG-36
Assignee:
Regents of University of California (Los Alamos, NM)
Patent Number(s):
US 5476764
OSTI ID:
870210
Country of Publication:
United States
Language:
English

References (7)

The role of hemoglobin denaturation and band 3 clustering in red blood cell aging journal February 1985
Hydroxyl radical formation by sickle erythrocyte membranes: role of pathologic iron deposits and cytoplasmic reducing agents journal November 1991
Increased susceptibility of stored erythrocytes to anti-band 3 IgG autoantibody binding journal August 1993
Rheologic properties of senescent erythrocytes: loss of surface area and volume with red blood cell age journal March 1992
The safety and therapeutic effectiveness of human red cells stored at - 80 degrees C for as long as 21 years journal June 1989
Decreased in vivo survival of hydrogen peroxide-damaged baboon red blood cells journal July 1990
Ascorbate protects against tert-butyl hydroperoxide inhibition of erythrocyte membrane Ca2+ + Mg2+-ATPase journal May 1990

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