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Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus

Patent ·
OSTI ID:869971
 [1];  [2];  [3];  [2]
  1. Littleton, CO
  2. Lakewood, CO
  3. Golden, CO
+ Show Author Affiliations

A purified low molecular weight .beta.-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-.beta.-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65.degree. C. at a pH range of from about 2 to about 7, has an inactivation temperature of about 80.degree. C. at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5-54.5 kD as determineded by SDS-PAGE.

Research Organization:
Midwest Research Institute
DOE Contract Number:
AC36-83CH10093
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Number(s):
US 5432075
OSTI ID:
869971
Country of Publication:
United States
Language:
English

References (5)

Mechanisms of Thermophily journal January 1978
Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases journal August 1989
Bacterium produces thermostable cellulase journal May 1987
The Production of Cellulases book June 1969
Cellulolytic enzyme system of Acetivibrio cellulolyticus journal March 1981

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Related Subjects

-d-glucopyranoside
-d-glucosidase
/435/
43068
5-54
50
65
80
acidothermus
acidothermus cellulolyticus
activity
atcc
beta
cellulolyticus
cellulolyticus atcc
degree
determineded
enzyme
exhibits
heat
inactivation
inactivation temperature
kd
molecular
molecular weight
optimal
ph
ph range
pnp-
possesses
produced
purified
range
sds-page
soluble
stability
temperature
temperature activity
thermostable
water
water soluble
weight