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Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068

Patent ·
OSTI ID:869113

A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068). The cellulase is water soluble, possesses both C.sub.1 and C.sub.x types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83.degree. C. at pH's from about 2 to about 9, and in inactivation temperature of about 110.degree. C. at pH's from about 2 to about 9.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO; Midwest Research Institute
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-83CH10093
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Number(s):
US 5275944
OSTI ID:
869113
Country of Publication:
United States
Language:
English

References (6)

Mechanisms of Thermophily journal January 1978
Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases journal August 1989
Bacterium produces thermostable cellulase journal May 1987
The Production of Cellulases book June 1969
Cellulolytic enzyme system of Acetivibrio cellulolyticus journal March 1981
Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria journal July 1986