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Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus

Patent ·
OSTI ID:868277
 [1];  [2];  [2];  [3]
  1. Lakewood, CO
  2. Littleton, CO
  3. Golden, CO
+ Show Author Affiliations

A substantially purified high molecular weight cellulase enzyme having a molecular weight of between about 156,000 to about 203,400 daltons isolated from the bacterium Acidothermus cellulolyticus (ATCC 43068) and a method of producing it are disclosed. The enzyme is water soluble, possesses both C.sub.1 and C.sub.x types of enzymatic activity, has a high degree of stability toward heat and exhibits both a high optimum temperature activity and high inactivation characteristics.

Research Organization:
MIDWEST RESEARCH INSTITUTE
DOE Contract Number:
AC02-83CH10093
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Number(s):
US 5110735
OSTI ID:
868277
Country of Publication:
United States
Language:
English

References (4)

Mechanisms of Thermophily journal January 1978
The Production of Cellulases book June 1969
Cellulolytic enzyme system of Acetivibrio cellulolyticus journal March 1981
Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria journal July 1986

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Related Subjects

/435/
000
156
203
400
43068
acidothermus
acidothermus cellulolyticus
activity
atcc
bacterium
cellulase
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cellulolyticus
characteristics
daltons
degree
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endoglucanase
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exhibits
heat
inactivation
isolated
method
molecular
molecular weight
optimum
optimum temperature
possesses
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purified
purified endoglucanas
purified endoglucanase
soluble
stability
substantially
temperature
temperature activity
thermophilic
thermostable
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types
water
water soluble
weight
weight cellulase