Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Detection and differentiation of coxiella burnetii in biological fluids

Patent ·
OSTI ID:868995
 [1];  [2];  [3];  [4]
  1. Richland, WA
  2. Moscow, ID
  3. Derwood, MD
  4. Albuquerque, NM
+ Show Author Affiliations

Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA with a DNA probe containing DNA sequences that specifically hybridize with C. burnetii DNA of strains associated with the capacity to cause acute or chronic disease.

Research Organization:
BATTELLE MEMORIAL INSTITUTE
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Number(s):
US 5258283
OSTI ID:
868995
Country of Publication:
United States
Language:
English

References (8)

Q fever and Coxiella burnetii: a model for host-parasite interactions journal June 1983
Chronic Q Fever: 1. Clinical Aspects of a Patient with Endocarditis journal November 1959
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. journal July 1983
Serological evaluation of O fever in humans: enhanced phase I titers of immunoglobulins G and A are diagnostic for Q fever endocarditis journal September 1983
Q fever endocarditis journal March 1982
Phase Variation of the nine mile and Other Strains of Rickettsia Burneti journal May 1956
Correlation of plasmid type and disease caused by Coxiella burnetii journal September 1985
Isolation and characterization of a plasmid from phase I Coxiella burnetii journal August 1983

Similar Records

Related Subjects

/435/436/999/
acute
acute disease
associated
biological
biological fluid
biological fluids
biological sample
biological samples
burnetii
capable
capacity
causing
causing acute
causing chronic
cells
cells contain
cells contained
cellular
cellular dna
chronic
chronic disease
comprise
comprise treating
contained
containing
coxiella
coxiella burnetii
detecting
detection
differentiating
differentiating strains
differentiation
disclosed
disease
dna
dna probe
dna sequence
dna sequences
expose
expose cellular
fluids
hybridize
hybridizing
method
methods
presence
probe
sample
samples
sequences
specifically
strains
strains capable
treating
treating cells