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Title: Detection and differentiation of coxiella burnetii in biological fluids

Patent ·
OSTI ID:868995
 [1];  [2];  [3];  [4]
  1. Richland, WA
  2. Moscow, ID
  3. Derwood, MD
  4. Albuquerque, NM
+ Show Author Affiliations

Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA with a DNA probe containing DNA sequences that specifically hybridize with C. burnetii DNA of strains associated with the capacity to cause acute or chronic disease.

Research Organization:
Battelle Memorial Institute, Columbus, OH (United States)
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Number(s):
US 5258283
OSTI ID:
868995
Country of Publication:
United States
Language:
English

References (8)

Chronic Q Fever: 1. Clinical Aspects of a Patient with Endocarditis journal November 1959
Q fever and Coxiella burnetii: a model for host-parasite interactions journal June 1983
Phase Variation of the nine mile and Other Strains of Rickettsia Burneti journal May 1956
Serological evaluation of O fever in humans: enhanced phase I titers of immunoglobulins G and A are diagnostic for Q fever endocarditis journal September 1983
Q fever endocarditis journal March 1982
Isolation and characterization of a plasmid from phase I Coxiella burnetii journal August 1983
Correlation of plasmid type and disease caused by Coxiella burnetii journal September 1985
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. journal July 1983

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detection
differentiation
coxiella
burnetii
biological
fluids
methods
detecting
presence
samples
method
differentiating
strains
capable
causing
acute
disease
chronic
disclosed
comprise
treating
cells
contained
sample
expose
cellular
dna
hybridizing
probe
containing
sequences
specifically
hybridize
associated
capacity
biological samples
dna sequences
dna sequence
cellular dna
biological fluids
treating cells
strains capable
biological fluid
comprise treating
acute disease
causing acute
causing chronic
coxiella burnetii
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cells contained
chronic disease
dna probe
expose cellular
differentiating strains
cells contain
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