Detection and differentiation of coxiella burnetii in biological fluids

Patent · Sun Jan 01 00:00:00 EST 1989

OSTI ID:867154

Frazier, Marvin E ^[1]; Mallavia, Louis P ^[2]; Baca, Oswald G ^[3]; Samuel, James E ^[4]

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1. Richland, WA
2. Moscow, ID
3. Albuquerque, NM
4. Pullman, WA

Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.

• Research Organization: Battelle Memorial Institute, Columbus, OH (United States)
• DOE Contract Number: AC06-76RL01830
• Assignee: Battelle Development Corporation (Columbus, OH)
• Patent Number(s): US 4876186
• OSTI ID: 867154
• Country of Publication: United States
• Language: English

Related Subjects

detection
differentiation
coxiella
burnetii
biological
fluids
methods
detecting
presence
samples
method
differentiating
strains
capable
causing
acute
disease
chronic
disclosed
comprise
treating
cells
contained
sample
expose
cellular
dna
hybridizing
specifically
rickettsial
burnetii-specific
labeled
probe
radioisotope
biotin
labels
preferred
allowing
autoradiography
colorimetric
assays
respectively
biological samples
labeled dna
cellular dna
biological fluids
treating cells
strains capable
biological fluid
comprise treating
acute disease
causing acute
causing chronic
colorimetric assays
coxiella burnetii
biological sample
cells contained
chronic disease
colorimetric assay
dna probe
expose cellular
differentiating strains
cells contain
/435/436/999/