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Rotor assembly and assay method

Patent ·
OSTI ID:868912
 [1];  [2];  [3]
  1. Oak Ridge, TN
  2. Loudon, TN
  3. Knoxville, TN
+ Show Author Affiliations

A rotor assembly for carrying out an assay includes a rotor body which is rotatable about an axis of rotation, and has a central chamber and first, second, third, fourth, fifth, and sixth chambers which are in communication with and radiate from the central chamber. The rotor assembly further includes a shuttle which is movable through the central chamber and insertable into any of the chambers, the shuttle including a reaction cup carrying an immobilized antigen or an antibody for transport among the chambers. A method for carrying out an assay using the rotor assembly includes moving the reaction cup among the six chambers by passing the cup through the central chamber between centrifugation steps in order to perform the steps of: separating plasma from blood cells, binding plasma antibody or antigen, washing, drying, binding enzyme conjugate, reacting with enzyme substrate and optically comparing the resulting reaction product with unreacted enzyme substrate solution. The movement of the reaction cup can be provided by attaching a magnet to the reaction cup and supplying a moving magnetic field to the rotor.

Research Organization:
LOCKHEED MARTIN ENRGY SYST INC
DOE Contract Number:
AC05-84OR21400
Assignee:
Martin Marietta Energy Systems, Inc. (Oak Ridge, TN)
Patent Number(s):
US 5242803
OSTI ID:
868912
Country of Publication:
United States
Language:
English

References (2)

Development of a simple device for processing whole-blood samples into measured aliquots of plasma. journal September 1986
Two-dimensional centrifugation for desk-top clinical chemistry. journal September 1985

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Related Subjects

/435/422/436/
antibody
antigen
assay
assay method
assembly
attaching
axis
binding
blood
blood cell
blood cells
carrying
cells
central
central chamber
centrifugation
chamber
chambers
communication
comparing
conjugate
cup
drying
enzyme
field
fifth
fourth
immobilized
including
insertable
magnet
magnetic
magnetic field
method
movable
movement
moving
optically
passing
perform
plasma
product
provided
radiate
reacting
reaction
reaction product
resulting
resulting reaction
rotatable
rotation
rotor
rotor assembly
separating
shuttle
six
sixth
solution
steps
substrate
supplying
third
transport
unreacted
washing