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Title: Rotor assembly and assay method

Abstract

A rotor assembly for carrying out an assay includes a rotor body which is rotatable about an axis of rotation, and has a central chamber and first, second, third, fourth, fifth, and sixth chambers which are in communication with and radiate from the central chamber. The rotor assembly further includes a shuttle which is movable through the central chamber and insertable into any of the chambers, the shuttle including a reaction cup carrying an immobilized antigen or an antibody for transport among the chambers. A method for carrying out an assay using the rotor assembly includes moving the reaction cup among the six chambers by passing the cup through the central chamber between centrifugation steps in order to perform the steps of: separating plasma from blood cells, binding plasma antibody or antigen, washing, drying, binding enzyme conjugate, reacting with enzyme substrate and optically comparing the resulting reaction product with unreacted enzyme substrate solution. The movement of the reaction cup can be provided by attaching a magnet to the reaction cup and supplying a moving magnetic field to the rotor. 34 figures.

Inventors:
; ;
Publication Date:
OSTI Identifier:
5440546
Patent Number(s):
US 5242803; A
Application Number:
PPN: US 7-878799
Assignee:
Martin Marietta Energy Systems, Inc., Oak Ridge, TN (United States) PTO; EDB-94-047385
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Resource Relation:
Patent File Date: 5 May 1992
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANTIBODIES; BIOASSAY; ANTIGENS; BLOOD PLASMA; SEPARATION PROCESSES; ENZYMES; SEPARATION EQUIPMENT; DESIGN; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; MATERIALS; ORGANIC COMPOUNDS; PROTEINS; 550200* - Biochemistry

Citation Formats

Burtis, C.A., Johnson, W.F., and Walker, W.A.. Rotor assembly and assay method. United States: N. p., 1993. Web.
Burtis, C.A., Johnson, W.F., & Walker, W.A.. Rotor assembly and assay method. United States.
Burtis, C.A., Johnson, W.F., and Walker, W.A.. Tue . "Rotor assembly and assay method". United States.
@article{osti_5440546,
title = {Rotor assembly and assay method},
author = {Burtis, C.A. and Johnson, W.F. and Walker, W.A.},
abstractNote = {A rotor assembly for carrying out an assay includes a rotor body which is rotatable about an axis of rotation, and has a central chamber and first, second, third, fourth, fifth, and sixth chambers which are in communication with and radiate from the central chamber. The rotor assembly further includes a shuttle which is movable through the central chamber and insertable into any of the chambers, the shuttle including a reaction cup carrying an immobilized antigen or an antibody for transport among the chambers. A method for carrying out an assay using the rotor assembly includes moving the reaction cup among the six chambers by passing the cup through the central chamber between centrifugation steps in order to perform the steps of: separating plasma from blood cells, binding plasma antibody or antigen, washing, drying, binding enzyme conjugate, reacting with enzyme substrate and optically comparing the resulting reaction product with unreacted enzyme substrate solution. The movement of the reaction cup can be provided by attaching a magnet to the reaction cup and supplying a moving magnetic field to the rotor. 34 figures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 07 00:00:00 EDT 1993},
month = {Tue Sep 07 00:00:00 EDT 1993}
}