Integrated fluorescence analysis system

Patent · 01 January 1992

OSTI ID:868318

Buican, Tudor N ^[1]; Yoshida, Thomas M ^[1]

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1. Los Alamos, NM

An integrated fluorescence analysis system enables a component part of a sample to be virtually sorted within a sample volume after a spectrum of the component part has been identified from a fluorescence spectrum of the entire sample in a flow cytometer. Birefringent optics enables the entire spectrum to be resolved into a set of numbers representing the intensity of spectral components of the spectrum. One or more spectral components are selected to program a scanning laser microscope, preferably a confocal microscope, whereby the spectrum from individual pixels or voxels in the sample can be compared. Individual pixels or voxels containing the selected spectral components are identified and an image may be formed to show the morphology of the sample with respect to only those components having the selected spectral components. There is no need for any physical sorting of the sample components to obtain the morphological information.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 5117466
• OSTI ID: 868318
• Country of Publication: United States
• Language: English

Related Subjects

/382/250/356/
analysis
birefringent
compared
component
components
confocal
confocal microscope
containing
cytometer
enables
entire
flow
flow cytometer
fluorescence
fluorescence analysis
fluorescence spectrum
formed
identified
image
individual
individual pixels
information
integrated
integrated fluorescence
intensity
laser
laser microscope
microscope
morphological
morphology
obtain
optics
physical
pixels
preferably
representing
resolved
respect
sample
sample components
sample volume
scanning
scanning laser
selected
selected spectral
set
sorted
sorting
spectral
spectral component
spectral components
spectrum
virtually
volume
voxels
whereby