Real-time Fourier transform spectrometry for fluorescence imaging and flow cytometry
We present a Fourier transform (FT) spectrometer that is suitable for real-time spectral analysis in fluorescence imaging and flow cytometry. The instrument consists of a novel type of interferometer that can be modulated at frequencies of up to 100 kHz and has a high light throughput; and a dedicated, parallel array processor for the real-time computation of spectral parameters. The data acquisition array processor can be programmed by a host computer to perform any desired linear transform on the interferogram and can thus separate contributions from multiple fluorescence microscopy. The integration of a flow cytometer and a spectral imaging fluorescence microscope is discussed, and the concepts of direct and reversed virtual sorting'' are introduced. 9 refs., 8 figs.
- Research Organization:
- Los Alamos National Lab., NM (USA)
- Sponsoring Organization:
- DOHHS
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 6906638
- Report Number(s):
- LA-UR-90-705; CONF-900140--20; ON: DE90008992
- Country of Publication:
- United States
- Language:
- English
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47 OTHER INSTRUMENTATION
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ARRAY PROCESSORS
BIOCHEMISTRY
BIOLOGY
CHEMISTRY
CYTOLOGY
DATA ACQUISITION SYSTEMS
EMISSION SPECTRA
EMISSION SPECTROSCOPY
FLUORESCENCE
FOURIER TRANSFORM SPECTROMETERS
FUNCTIONS
IMAGE PROCESSING
LUMINESCENCE
MEASURING INSTRUMENTS
PROCESSING
RESPONSE FUNCTIONS
SIGNAL CONDITIONING
SPECTRA
SPECTROMETERS
SPECTROSCOPY