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Title: Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus

Patent ·
OSTI ID:868277
 [1];  [2];  [2];  [3]
  1. Lakewood, CO
  2. Littleton, CO
  3. Golden, CO
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A substantially purified high molecular weight cellulase enzyme having a molecular weight of between about 156,000 to about 203,400 daltons isolated from the bacterium Acidothermus cellulolyticus (ATCC 43068) and a method of producing it are disclosed. The enzyme is water soluble, possesses both C.sub.1 and C.sub.x types of enzymatic activity, has a high degree of stability toward heat and exhibits both a high optimum temperature activity and high inactivation characteristics.

Research Organization:
Midwest Research Institute, Kansas City, MO (United States)
DOE Contract Number:
AC02-83CH10093
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Number(s):
US 5110735
OSTI ID:
868277
Country of Publication:
United States
Language:
English

References (4)

Cellulolytic enzyme system of Acetivibrio cellulolyticus journal March 1981
The Production of Cellulases book June 1969
Mechanisms of Thermophily journal January 1978
Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria journal July 1986

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Related Subjects

thermostable
purified
endoglucanase
thermophilic
bacterium
acidothermus
cellulolyticus
substantially
molecular
weight
cellulase
enzyme
156
000
203
400
daltons
isolated
atcc
43068
method
producing
disclosed
water
soluble
possesses
types
enzymatic
activity
degree
stability
heat
exhibits
optimum
temperature
inactivation
characteristics
cellulase enzyme
optimum temperature
water soluble
molecular weight
acidothermus cellulolyticus
thermostable purified
temperature activity
purified endoglucanase
enzymatic activity
weight cellulase
purified endoglucanas
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