Cloning and expression of the gene for bacteriophage T7 RNA polymerase

Patent · Sun Dec 31 23:00:00 EST 1989

OSTI ID:867506

Studier, F William ^[1]; Davanloo, Parichehre ^[2]; Rosenberg, Alan H ^[3]; Moffatt, Barbara A ^[4]; Dunn, John J ^[5]

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1. Stony Brook, NY
2. Basel, CH
3. Setauket, NY
4. East Lansing, MI
5. Bellport, NY

This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the T7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.

• Research Organization: ASSOC UNIVERSITIES INC
• DOE Contract Number: AC02-76CH00016
• Assignee: Associated Universities, Inc. (Washington, DC)
• Patent Number(s): US 4952496
• OSTI ID: 867506
• Country of Publication: United States
• Language: English

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