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Title: Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof

Abstract

The present invention provides monoclonal antibodies specific to and distinguish between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype.

Inventors:
 [1];  [2];  [1];  [1];  [3];  [4]
  1. (Livermore, CA)
  2. (San Ramon, CA)
  3. (Walnut Creek, CA)
  4. (Berkeley, CA)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
866631
Patent Number(s):
US 4752583
Assignee:
United States of America as represented by United States (Washington, DC) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
monoclonal; antibodies; human; hemoglobin; cell; lines; production; provides; specific; distinguish; methods; capable; distinguishing; types; single; amino; acid; residue; produced; according; method; useful; immunofluorescent; markers; enumerate; circulating; blood; cells; property; altered; expression; due; somatic; mutation; measurement; contemplated; assay; vivo; cellular; mutations; humans; accordance; instant; exhibit; degree; specificity; affinity; suitable; labeling; flow; cytometric; detection; genotype; produced according; altered expression; cell lines; blood cells; amino acid; monoclonal antibodies; flow cytometric; blood cell; fluorescent markers; antibodies specific; acid residue; cell line; /530/435/436/930/

Citation Formats

Jensen, Ronald H., Vanderlaan, Martin, Bigbee, William L., Stanker, Larry H., Branscomb, Elbert W., and Grabske, Robert J.. Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof. United States: N. p., 1988. Web.
Jensen, Ronald H., Vanderlaan, Martin, Bigbee, William L., Stanker, Larry H., Branscomb, Elbert W., & Grabske, Robert J.. Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof. United States.
Jensen, Ronald H., Vanderlaan, Martin, Bigbee, William L., Stanker, Larry H., Branscomb, Elbert W., and Grabske, Robert J.. Fri . "Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof". United States. https://www.osti.gov/servlets/purl/866631.
@article{osti_866631,
title = {Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof},
author = {Jensen, Ronald H. and Vanderlaan, Martin and Bigbee, William L. and Stanker, Larry H. and Branscomb, Elbert W. and Grabske, Robert J.},
abstractNote = {The present invention provides monoclonal antibodies specific to and distinguish between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}

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