Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof

Patent · Fri Jan 01 00:00:00 EST 1988

OSTI ID:866631

Jensen, Ronald H ^[1]; Vanderlaan, Martin ^[2]; Bigbee, William L ^[1]; Stanker, Larry H ^[1]; Branscomb, Elbert W ^[3]; Grabske, Robert J ^[4]

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1. Livermore, CA
2. San Ramon, CA
3. Walnut Creek, CA
4. Berkeley, CA

The present invention provides monoclonal antibodies specific to and distinguish between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4752583
• OSTI ID: 866631
• Country of Publication: United States
• Language: English

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/530/435/436/930/