Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof
The present invention provides monoclonal antibodies specific to and distinguishing between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype. 4 figs.
- Research Organization:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- DOE Contract Number:
- W-7405-ENG-48
- Assignee:
- Dept. of Energy
- Application Number:
- ON: DE86013864
- OSTI ID:
- 5577044
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HEMOGLOBIN
ANTIGEN-ANTIBODY REACTIONS
HYBRIDOMAS
DESIGN
SICKLE CELL ANEMIA
DIAGNOSTIC TECHNIQUES
CELL FLOW SYSTEMS
FLUORESCENCE
MONOCLONAL ANTIBODIES
ANEMIAS
ANIMAL CELLS
ANTIBODIES
CARBOXYLIC ACIDS
DISEASES
GLOBIN
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LUMINESCENCE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
SYMPTOMS
550900* - Pathology