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Title: Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof

Abstract

The present invention provides monoclonal antibodies specific to and distinguishing between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype. 4 figs.

Inventors:
; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab., CA (USA)
OSTI Identifier:
5577044
Application Number:
ON: DE86013864
Assignee:
Dept. of Energy LLNL; ERA-11-005628; EDB-86-138824
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HEMOGLOBIN; ANTIGEN-ANTIBODY REACTIONS; HYBRIDOMAS; DESIGN; SICKLE CELL ANEMIA; DIAGNOSTIC TECHNIQUES; CELL FLOW SYSTEMS; FLUORESCENCE; MONOCLONAL ANTIBODIES; ANEMIAS; ANIMAL CELLS; ANTIBODIES; CARBOXYLIC ACIDS; DISEASES; GLOBIN; HEMIC DISEASES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; LUMINESCENCE; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PIGMENTS; PORPHYRINS; PROTEINS; SYMPTOMS; 550900* - Pathology

Citation Formats

Jensen, R.H., Vanderlaan, M., Bigbee, W.L., Stanker, L.H., Branscomb, E.W., and Grabske, R.J. Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof. United States: N. p., 1984. Web.
Jensen, R.H., Vanderlaan, M., Bigbee, W.L., Stanker, L.H., Branscomb, E.W., & Grabske, R.J. Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof. United States.
Jensen, R.H., Vanderlaan, M., Bigbee, W.L., Stanker, L.H., Branscomb, E.W., and Grabske, R.J. Thu . "Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof". United States.
@article{osti_5577044,
title = {Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof},
author = {Jensen, R.H. and Vanderlaan, M. and Bigbee, W.L. and Stanker, L.H. and Branscomb, E.W. and Grabske, R.J.},
abstractNote = {The present invention provides monoclonal antibodies specific to and distinguishing between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Nov 29 00:00:00 EST 1984},
month = {Thu Nov 29 00:00:00 EST 1984}
}