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Title: Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds

Abstract

Apparatus and method for electronically reading planar two dimensional .beta.-ray emitter-labeled gel electrophoretograms. A single, flat rectangular multiwire proportional chamber is placed in close proximity to the gel and the assembly placed in an intense uniform magnetic field disposed in a perpendicular manner to the rectangular face of the proportional chamber. Beta rays emitted in the direction of the proportional chamber are caused to execute helical motions which substantially preserve knowledge of the coordinates of their origin in the gel. Perpendicularly oriented, parallel wire, parallel plane cathodes electronically sense the location of the .beta.-rays from ionization generated thereby in a detection gas coupled with an electron avalanche effect resulting from the action of a parallel wire anode located therebetween. A scintillator permits the present apparatus to be rendered insensitive when signals are generated from cosmic rays incident on the proportional chamber. Resolution for concentrations of radioactive compounds in the gel exceeds 700 .mu.m. The apparatus and method of the present invention represent a significant improvement over conventional autoradiographic techniques in dynamic range, linearity and sensitivity of data collection. A concentration and position map for gel electrophoretograms having significant concentrations of labeled compounds and/or highly radioactive labeling nuclides can generally bemore » obtained in less than one hour.« less

Inventors:
 [1];  [2];  [2]
  1. Santa Fe, NM
  2. Los Alamos, NM
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
OSTI Identifier:
866329
Patent Number(s):
US 4686368
Assignee:
United States of America as represented by United States (Washington, DC)
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
apparatus; method; reading; two-dimensional; electrophoretograms; containing; beta; -ray-emitting; labeled; compounds; electronically; planar; dimensional; -ray; emitter-labeled; single; flat; rectangular; multiwire; proportional; chamber; placed; close; proximity; assembly; intense; uniform; magnetic; field; disposed; perpendicular; manner; rays; emitted; direction; caused; execute; helical; motions; substantially; preserve; knowledge; coordinates; origin; perpendicularly; oriented; parallel; wire; plane; cathodes; sense; location; -rays; ionization; generated; detection; gas; coupled; electron; avalanche; effect; resulting; action; anode; located; therebetween; scintillator; permits; rendered; insensitive; signals; cosmic; incident; resolution; concentrations; radioactive; exceeds; 700; represent; significant; improvement; conventional; autoradiographic; techniques; dynamic; range; linearity; sensitivity; data; collection; concentration; position; map; highly; labeling; nuclides; obtained; hour; uniform magnetic; beta rays; wire anode; dynamic range; close proximity; magnetic field; data collection; electron avalanche; radioactive compound; beta ray; labeled compounds; located therebetween; parallel wire; perpendicular manner; active compound; rays emitted; significant improvement; cosmic rays; significant concentration; rays incident; /250/

Citation Formats

Anderson, Herbert L, Kinnison, W Wayne, and Lillberg, John W. Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds. United States: N. p., 1987. Web.
Anderson, Herbert L, Kinnison, W Wayne, & Lillberg, John W. Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds. United States.
Anderson, Herbert L, Kinnison, W Wayne, and Lillberg, John W. Thu . "Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds". United States. https://www.osti.gov/servlets/purl/866329.
@article{osti_866329,
title = {Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds},
author = {Anderson, Herbert L and Kinnison, W Wayne and Lillberg, John W},
abstractNote = {Apparatus and method for electronically reading planar two dimensional .beta.-ray emitter-labeled gel electrophoretograms. A single, flat rectangular multiwire proportional chamber is placed in close proximity to the gel and the assembly placed in an intense uniform magnetic field disposed in a perpendicular manner to the rectangular face of the proportional chamber. Beta rays emitted in the direction of the proportional chamber are caused to execute helical motions which substantially preserve knowledge of the coordinates of their origin in the gel. Perpendicularly oriented, parallel wire, parallel plane cathodes electronically sense the location of the .beta.-rays from ionization generated thereby in a detection gas coupled with an electron avalanche effect resulting from the action of a parallel wire anode located therebetween. A scintillator permits the present apparatus to be rendered insensitive when signals are generated from cosmic rays incident on the proportional chamber. Resolution for concentrations of radioactive compounds in the gel exceeds 700 .mu.m. The apparatus and method of the present invention represent a significant improvement over conventional autoradiographic techniques in dynamic range, linearity and sensitivity of data collection. A concentration and position map for gel electrophoretograms having significant concentrations of labeled compounds and/or highly radioactive labeling nuclides can generally be obtained in less than one hour.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1987},
month = {1}
}

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