Automated apparatus for producing gradient gels

Patent · Tue Dec 31 23:00:00 EST 1985

OSTI ID:865874

Anderson, Norman L ^[1]

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1. Clarendon Hills, IL

Apparatus for producing a gradient gel which serves as a standard medium for a two-dimensional analysis of proteins, the gel having a density gradient along its height formed by a variation in gel composition, with the apparatus including first and second pumping means each including a plurality of pumps on a common shaft and driven by a stepping motor capable of providing small incremental changes in pump outputs for the gel ingredients, the motors being controlled, by digital signals from a digital computer, a hollow form or cassette for receiving the gel composition, means for transferring the gel composition including a filler tube extending near the bottom of the cassette, adjustable horizontal and vertical arms for automatically removing and relocating the filler tube in the next cassette, and a digital computer programmed to automatically control the stepping motors, arm movements, and associated sensing operations involving the filling operation.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL
• DOE Contract Number: W-31109-ENG-38
• Assignee: United States of America as represented by United States ()
• Patent Number(s): US 4594064
• OSTI ID: 865874
• Country of Publication: United States
• Language: English

Related Subjects

/425/204/
adjustable
analysis
apparatus
apparatus including
arm
arms
associated
automated
automated apparatus
automatically
automatically control
bottom
capable
cassette
changes
common
common shaft
composition
composition including
computer
control
controlled
density
density gradient
digital
digital computer
digital signal
digital signals
driven
extending
filler
filling
form
formed
gels
gradient
height
hollow
horizontal
including
incremental
ingredients
involving
means
medium
motor
motor capable
motors
movements
near
operation
operations
outputs
plurality
producing
programmed
proteins
providing
pump
pumping
pumping means
pumps
receiving
relocating
removing
sensing
serves
shaft
signals
standard
stepping
stepping motor
transferring
tube
tube extending
two-dimensional
variation
vertical