Electronic method for autofluorography of macromolecules on two-D matrices

Patent · Sat Jan 01 04:00:00 EST 1983

OSTI ID:864592

Davidson, Jackson B ^[1]; Case, Arthur L ^[1]

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1. Oak Ridge, TN

A method for detecting, localizing, and quantifying macromolecules contained in a two-dimensional matrix is provided which employs a television-based position sensitive detection system. A molecule-containing matrix may be produced by conventional means to produce spots of light at the molecule locations which are detected by the television system. The matrix, such as a gel matrix, is exposed to an electronic camera system including an image-intensifier and secondary electron conduction camera capable of light integrating times of many minutes. A light image stored in the form of a charge image on the camera tube target is scanned by conventional television techniques, digitized, and stored in a digital memory. Intensity of any point on the image may be determined from the number at the memory address of the point. The entire image may be displayed on a television monitor for inspection and photographing or individual spots may be analyzed through selected readout of the memory locations. Compared to conventional film exposure methods, the exposure time may be reduced 100-1000 times.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4389670
• OSTI ID: 864592
• Country of Publication: United States
• Language: English

Related Subjects

/348/250/376/
100-1000
address
analyzed
autofluorography
camera
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camera tube
capable
charge
compared
conduction
contained
conventional
conventional means
detected
detecting
detection
determined
digital
digitized
dimensional matrix
displayed
electron
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electronic
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employs
entire
exposed
exposure
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film
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image
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integrating
intensity
light
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light integrating
localizing
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macromolecules
matrices
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memory
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method
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minutes
molecule
molecule-containing
monitor
photographing
position
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produce
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reduced
scanned
secondary
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spots
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target
techniques
television
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